Our Release of Glycans service is designed to provide highly efficient, reproducible, and precise liberation of glycans from complex glycoproteins and glycolipids as part of our comprehensive Glycan Profile analysis. By applying both enzymatic and chemical strategies, we ensure that N-glycans and O-glycans are released under optimal conditions, preserving their native structures for downstream labeling, detection, and characterization. This service is suitable for biopharmaceutical research, biomarker discovery, and quality control applications, giving researchers and developers reliable access to accurate glycan data that supports deeper structural insights and robust decision-making.
Complex Glycan Structures: Native glycans are often hidden or bound within glycoproteins, making direct profiling difficult. Our release service ensures glycans are efficiently liberated for analysis.
Inconsistent Results: Manual or non-standardized glycan release methods often yield incomplete or biased outcomes. Our optimized process eliminates these inconsistencies.
Limited Research Insights: Without proper glycan release, researchers may overlook critical structural variations. We help maximize glycomics data reliability and depth.
Time and Resource Constraints: Developing in-house protocols for glycan release can be costly and time-consuming. Our service provides ready-to-use, expert solutions.
We apply specific glycosidases such as PNGase F to release glycans with high efficiency while preserving structural integrity.
Our chemical release methods offer flexibility for glycans not accessible via enzymatic digestion.
We prepare samples to ensure glycans are released in pure, stable, and analyzable form.
Our service supports projects of varying sizes, from early-stage research to industrial-scale studies.
We ensure a seamless transition from glycan release to advanced glycan profiling and structural analysis.
We deliver clear, detailed documentation to support both research and regulatory submissions.
Our Release of Glycans service is compatible with a wide range of biological and biopharmaceutical samples, ensuring flexibility and reliability for different research and industrial applications.
Partnering with us for glycan release ensures:
High Accuracy: Validated protocols deliver precise and reproducible glycan release.
Wide Compatibility: Suitable for biopharmaceuticals, cells, biological fluids, and gel samples.
End-to-End Support: Comprehensive service from consultation to data reporting.
Time & Cost Efficiency: Ready-to-use solutions reduce in-house workload and expenses.
Regulatory-Ready: Documentation tailored for research, industrial, and compliance needs.
Our Release of Glycans service follows a streamlined and standardized workflow to ensure high-quality, reproducible results:
Clients provide detailed sample information and research objectives.
Our team advises on the most suitable glycan release strategy.
Removal of interfering components and stabilization of sensitive molecules.
Tailored pretreatment for biopharmaceuticals, cells, biological fluids, or gel-embedded glycoproteins.
Enzymatic digestion (e.g., PNGase F) for N-glycans.
Chemical release strategies for O-glycans or complex targets.
Optimized conditions to preserve structural fidelity.
Purification and stabilization of released glycans.
Optional labeling for enhanced detection.
Seamless transfer to downstream analysis (LC-MS, HPLC, CE).
Data generation for comprehensive glycomic insights.
Detailed documentation of protocols and findings.
Results delivered in formats suitable for research, regulatory, or industrial applications.
We accept biopharmaceuticals, mammalian and bacterial cells, biological fluids, tissues, and glycoproteins in SDS-gel (N-glycans only).
Enzymatic release (e.g., PNGase F) is preferred for N-glycans, while chemical methods are used for O-glycans or complex glycoproteins. We recommend the most suitable strategy based on sample type and study goals.
Released glycans can be labeled and analyzed using HPLC, LC-MS, or CE, seamlessly integrated with our Glycan Profile service.
The required amount depends on the sample type and research purpose, but we provide flexible solutions from microgram to milligram levels.
Partner with us to accelerate your research and development through our Release of Glycans service integrated within Glycan Profile analysis. Our expert team not only provides reliable glycan release and profiling workflows, but also offers access to a range of well-characterized glycans, including high-mannose glycans, complex-type N-glycans, hybrid glycans, and core O-glycans. These reference standards and custom glycan panels can support method development, validation, and advanced comparative studies.
Contact us today to discuss your project needs and discover how our glycan solutions can enhance your biopharmaceutical development, clinical research, or glycobiology studies.
