We provide advanced Glycan Engineering services that empower biopharma companies, research institutes, and innovators to precisely control protein and cell glycosylation. By combining gene editing-based glycosylation pathway design, metabolic engineering with sugar analogs, and state-of-the-art glycomics analysis, we enable the development of next-generation biologics, vaccines, and cell therapies with improved stability, efficacy, and safety. Our expertise covers the entire process-from early-stage design and proof-of-concept studies to custom glycoprotein production and biologics optimization-ensuring reliable results and seamless translation from discovery to commercialization.
Inconsistent Glycosylation in Biopharmaceuticals - Standard expression systems often produce heterogeneous glycans, reducing drug stability and efficacy.
Short Half-Life of Therapeutic Proteins - Unoptimized glycans accelerate clearance from circulation.
Immune Recognition & Safety Risks - Unwanted glycan structures can trigger immunogenicity, complicating therapeutic approval.
Limited Vaccine Efficacy - Without proper glycan design, antigens fail to mimic native pathogens, weakening immune responses.
Lack of Customization for Research - Researchers need tailor-made glycan modifications to investigate disease pathways and cell signaling.
Insufficient Drug Efficacy - By precisely modulating antibody glycosylation structures (e.g., Fc glycoforms), therapeutic performance can be improved through enhanced ADCC/CDC activity or extended half-life.
Challenges in Target Validation - Using MOE (Metabolic Oligosaccharide Engineering) technology to label tumor-associated carbohydrate antigens (such as Tn/sTn) provides a powerful approach to accelerate targeted drug development.
1. Metabolic Oligosaccharide Engineering (MOE)
Service: Design of unnatural sugars, optimization of cellular uptake, bioorthogonal labeling, and in vivo imaging.
Application: Enables glycan labeling, vaccine development, and tumor-associated carbohydrate antigen (TACA) research.
2. Glycosyltransferase Engineering
Service: Directed evolution of glycosyltransferases, glycoform remodeling, and site-specific glycosylation.
Application: Supports antibody drug optimization and consistency control of recombinant proteins.
3. Knockout/Knock-in Gene Editing
Service: Targeted knockout or knock-in of glycosylation-related genes using CRISPR-Cas9, combined with reprogramming of glycosynthetic pathways to achieve precise glycan control.
Application: Enables cell line development and functional studies of glycan biosynthesis and regulation.
4. Host Cell Engineering
We develop tailored cell platforms (CHO, HEK293, yeast, and E. coli) optimized for desired glycan expression.
1. Glycan Structural Characterization
Service: Comprehensive profiling by MALDI-TOF, LC-MS, GC-MS, and NMR spectroscopy.
Application: Provides glycoform identification, structural confirmation, and batch-to-batch comparison.
2. Glycosylation Site Mapping
Service: Enzymatic digestion combined with mass spectrometry, ETD fragmentation, and occupancy quantification.
Application: Ensures glycopeptide localization, quality control, and regulatory submission data.
3. Glycan Heterogeneity Analysis
Service: Quantitative profiling using 2D-HPLC, CE-LIF, and HILIC-MS.
Application: Evaluates batch consistency and process stability.
4. Glycan Antigen Epitope Screening
Service: Glycan microarray-antibody hybridization, phage display, and affinity ranking.
Application: Supports tumor-associated carbohydrate antigen discovery and vaccine target selection.
1. Antibody Glycoform Optimization
Service: Precise control of Fc defucosylation, mannosylation, and sialylation.
Application: Enhances ADCC/CDC activity, extends half-life, and reduces immunogenicity.
2. Glycan-Based Vaccine Development
Service: Synthesis and protein conjugation of Tn, STn, Globo H, and other glycan antigens, with immunogenicity testing.
Application: Advances therapeutic cancer vaccines and infectious disease vaccine candidates.
3. Glycan-Drug Conjugates (GDC)
Service: Use of glycans as targeting carriers conjugated with toxins/small molecules and linker design.
Application: Enables targeted chemotherapy and ADC alternatives.
4. Glycan Immunogenicity Assessment
Service: Anti-glycan antibody ELISA, Luminex assays, and T-cell activation studies.
Application: Supports preclinical safety evaluation and ADA prediction.
1. Glyco-Controlled Upstream Process Development
Service: Optimization through medium formulation DoE, glycosylation enzyme induction, and feeding strategies.
Application: Achieves consistent glycoforms, improved yield, and reduced cost.
2. GMP Glycoform Consistency Verification
Service: Statistical analysis of multi-batch glycan profiles, release standard setting, and ICH-compliant templates.
Application: Supports IND/BLA submission and regulatory inspections.
3. Glycan Patent Mining and Strategy
Service: Novel glycoform patent search, claim design, FTO analysis, and global filing strategies.
Application: Ensures IP protection, builds competitive barriers, and enables technology transfer.
Partnering with us for Glycan Engineering offers multiple benefits:
Scientific Precision: Our dual expertise in CRISPR gene editing and metabolic glycan engineering ensures unmatched control over glycosylation pathways.
Custom-Tailored Solutions: We deliver client-specific glycan profiles - from homogeneous therapeutic antibodies to unique sugar analog incorporation for diagnostics.
End-to-End Capabilities: From design to scale-up, we manage every stage, reducing time-to-market for biopharmaceutical products.
Innovation-Driven Approach: We combine synthetic biology, chemical biology, and advanced analytics to push the boundaries of what glycan engineering can achieve.
Our Glycan Engineering services are versatile and impact multiple areas across biotechnology, pharmaceuticals, and research:
We follow a structured, transparent workflow to ensure every project is delivered with scientific precision and commercial value:
Understand client goals, therapeutic targets, or research needs.
Define required glycosylation patterns and technical feasibility.
Select optimal host system (CHO, HEK293, microbial, or custom).
Develop glycosylation modification plan using gene editing or sugar analog pathways.
Perform precise genetic modifications or metabolic glycan engineering.
Monitor expression systems for desired glycoforms.
Apply LC-MS, HPLC, and glycomics profiling to confirm glycan structures.
Conduct functional assays (e.g., stability, ADCC, immunogenicity).
Continuous optimization and consulting.
Long-term support for regulatory compliance and commercialization.
Provide purified, validated proteins or engineered cells.
Offer process scale-up guidance for preclinical development.
Glycan Engineering is the precise modification of protein and cell surface glycans using gene editing or metabolic sugar analogs. It is critical for creating safer, more effective biopharmaceuticals and unlocking new research insights.
Our services are tailored for biopharma companies, vaccine developers, diagnostic innovators, and academic research labs.
Every project undergoes glycan profiling, LC-MS/HPLC analysis, and functional validation to guarantee reproducibility and compliance with regulatory standards.
Simply contact our expert team with your project requirements. We'll provide a tailored roadmap, from design to delivery.
Unlock the full potential of glycosylation science with our tailored services. Whether you need optimized therapeutic antibodies, glyco-enhanced vaccines, or engineered cells for advanced research, our team delivers solutions that combine precision, scalability, and innovation.
In addition to engineering pathways, we also provide ready-to-use glycans that can be directly conjugated to proteins, antibodies, or nanomaterials. These conjugation-ready glycans accelerate your R&D timelines by enabling rapid functionalization and validation without the need for lengthy in-house synthesis.
Start your project today - contact our expert team to discuss your specific needs and discover how our Glycan Engineering platform can drive your innovation forward.
