Liquid chromatography–tandem mass spectrometry method for the analysis of N-(3-aminopropyl)-N-dodecylpropane-1, 3-diamine, a biocidal disinfectant, in dairy products

Slimani, K., Pirotais, Y., Maris, P., Abjean, J. P., & Hurtaud-Pessel, D.

Food chemistry 262 (2018): 168-177.

A novel and reliable method to quantify residual levels of N-(3-aminopropyl)-N-dodecylpropane-1, 3-diamine in dairy products using ion-pairing reversed-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and fully validated. Sample extraction was done with salting-out technique using acetonitrile and sodium chloride. For LC-MS/MS, the analyte was detected using positive electrospray ionization (ESI+) and two multiple reaction monitoring (MRM) transitions were monitored. The method was validated in the 5-150 µg kg-1 range using total error approach. Thus, performance criteria of the method were evaluated. Relative standard deviations for trueness and precision were lower than 10%; with the exception of hard pressed cheese at 5 µg kg−1 for precision. The limit of quantification (LOQ) was around 5-7 µg kg-1 depending on the matrix of interest. The method was successfully applied to accurately quantify N-(3-aminopropyl)-N-dodecylpropane-1, 3-diamine in 146 various dairy products with a maximum contamination level of 225 µg kg-1 in cheese.


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