1. The simultaneous determination of berberine, palmatine, coptisine, epiberberine and jatrorrhizine in rat plasma by LC-MS/MS and a pharmacokinetic comparison after the oral administration of Rhizoma coptidis and Jiao-Tai-Wan extract
Guanghui Liu, Wei He, Hao Cai, Zhiyong Xie* and Qiongfeng Liao*. Anal. Methods,2014, 6, 2998–3008
The mass spectrometer was operated in the positive ion mode using multiple reaction monitoring (MRM) to detect the mass transitions. Both analytes and IS were detected by tandem mass spectrometry using the MRM of the precursor–product ion transitions at m/z 336.0 → 320.1 and m/z 336.0 → 292.1 for berberine, at m/z 352.0 → 336.1 and m/z 352.0 → 308.1 for palmatine, at m/z 320.0 → 292.1 and m/z 320.0 → 277.1 for coptisine, at m/z 336.0 → 320.1 and m/z 336.0 → 292.1 for epiberberine, at m/z 338.0 → 322.1 and m/z 338.0 → 294.1 for jatrorrhizine, and at m/z 356.4 → 192.1 and m/z 356.4 → 176.1 for tetrahydropalmatine. The MRM transitions m/z 336.0 → 320.1, m/z 352.0 → 336.1, m/z 338.0 → 322.1, m/z 336.0 → 320.1, m/z 320.0 →292.1 and m/z 356.4 → 192.1were selected for determining berberine, palmatine, jatrorrhizine, epiberberine, coptisine and tetrahydropalmatine, respectively. Theotherone(m/z 336.0 → 292.1, m/z 352.0 → 308.1, m/z 338.0 → 294.1, m/z 336.0 → 292.1, m/z 320.0 → 277.1 and m/z 356.4 → 176.1 for the analysis of berberine, palmatine, jatrorrhizine, epiberberine, coptisine and tetrahydropalmatine, respectively) was used as a qualitative channel to confirm the identity of berberine, palmatine, jatrorrhizine, epiberberine, coptisine and tetrahydropalmatine.
2. Determination of newly synthesized lipoic acidfasudil dimer in rat plasma by LC-MS/MS and its application to pharmacokinetics study
Waner Hou, Guanghui Liu, Xiuman Sun, Zhiyong Xie,* Qiongfeng Liao*. Anal. Methods,2014, 6,8675–8681
In the positive ion full scan mode, the protonated molecular ions [M + H] + for L–F 001 and tetrahydropalmatine were the most abundant ions. The operation parameters, including fragmentor and collision energy, were optimized by Mass-Hunter Optimizer software. The MS/MS product ion spectra of L–F 001 and IS are shown in Fig. 2. Under the product ion scan mode, the most intensive product ions observed were m/z 480.1 → 189.0 for L–F 001 and m/z 356.4 → 192.1 for IS. Therefore, they were selected for the determination of L–F 001 and tetrahydropalmatine. For confirming the identity of L–F 001 and IS, two more ion transitions, m/z 480.1 → 161.0 for L–F 001 and m/z 356.4 → 176.1 for IS, were used as qualitative channels.
3. Antihypertensive and vasorelaxant effects of Rhizoma corydalis and its active component tetrahydropalmatine via NO/cGMP pathway and calcium channel blockade in isolated rat thoracic aorta
Zhuo Qu, Jingze Zhang, Wenyuan Gao*. RSC Adv.,2015, 5, 94130–94143
Rhizoma corydalis has been used for the treatment of various cardiovascular diseases in China. It is also a well-known traditional Chinese herb for the treatment of chest pain, epigastria pain, dysmenorrhea, traumatic swelling and pain. Tetrahydropalmatine (THP, the structure shown in Fig. 1), one of its main active ingredients, has been demonstrated to have potent analgesic eﬀects and has been used in Chinese clinical practice for many years. It has various pharmacology eﬀects including antihypertensive activity, protective eﬀects towards human endothelial cells, anxiolytic properties, protection against methamphetamine-induced spatial learning, memory impairment and protection against myocardial ischaemia-reperfusion injury. Dehydrocorydaline, another important ingredient, demonstrates a relaxant eﬀect on thoracic aorta with intact or without endothelium. Although there is a lot of research about the cardiovascular protective eﬀects of Rhizoma corydalis and Tetrahydropalmatine (THP), their vasodilatory eﬀects have not yet been defined. Therefore, the aim of the present study was to investigate the possibility and to delineate the possible mechanisms of vasodilatation induced by RC and Tetrahydropalmatine (THP) on isolated rat aorta.