Fluorescent Enzyme Substrates

Ac-ANW-AMC
Bz-FVR-AMC
C12FDGlcU
CYP3A4 Green
DEPFMU
Dnp-PLALWAR
Dnp-PLAYWAR
Dnp-PLGLWA-DArg-NH2
Dnp-PLGMWSR

Background


Research and exploration in the microscopic world of cells and genes, genetic reporter genes are very useful “visualization and quantification” tools, with a wide range of applications. Luciferase is an ideal reporter gene for its excellent sensitivity, ease of use, and quantitative detection. Luciferase is not a specific molecule. It is a general term for enzymes that catalyze the production of bioluminescence. Different sources of luciferase have their own characteristics, which can catalyze the emission of different colors of light from substrates, and some can also detect two-color luminescence.

Fluorescent Enzyme Substrates

Application of Fluorescent Enzyme

  • Analysis of cis-acting elements and trans-acting factors in promoter studies
  • Drug screening
  • siRNA and miRNA screening
  • Secretory pathway and protein localization reporter gene detection
  • Real-time dynamic study of living cells
  • Signal transduction pathway analysis
  • Studies of difficult-to-transfect cells (including stem cells and primary cells)
  • RNA plicing studies

Below is a list of our available luciferase for cell structure and organelle (include but not limited to the following):

  • Gaussia luciferase

Gaussia luciferase, a secreted blue luciferase with a smaller molecular weight of only 22KD, contains a natural secretory signal peptide. This signal peptide directs the secretion of luciferase into the cell culture supernatant, allowing detection of reporter gene activity without lysing the cells. Secretory expression brings great convenience for detection: real-time kinetic analysis of live cells and continuous time curve study can be performed without lysis of precious cells. It can be combined with red firefly luciferase as a two-color detection system.

  • Cypridina luciferase

Cypridina luciferase is also a secreted luciferase with a beautiful blue-purple color and a protein molecular weight of 62KD. Like Gaussia, most of the luciferase products are secreted extracellularly, allowing continuous detection of live cells, which is very convenient. Due to the strong signal, residual luciferase in the cell is sufficient for routine lytic cell detection, enabling multiple assays. Cypridina luciferase can be combined with red firefly luciferase for two-color detection.

  • Green Renilla (green sea kidney) luciferase

Green Renilla (green sea kidney) luciferase is an intracellular protein that is more stable and more radiant in serum than the natural Renilla luciferase. Therefore, the use of the Green Renilla luciferase reporter gene is more sensitive.

Conclusion

Fluorescent enzyme can be used as a single reporter gene to study a biological event in a specific biological experiment. Since the luminescence spectrum characteristics and substrates of different luciferases are different, it is also possible to use a plurality of different fluorescent enzyme in combination for multiplex detection. BOC Sciences can provides wide range of related fluorescent enzyme substrates to support your regulatory, development or research programs.

References

1. Nguyen, T. G. , Honson, N. S. , Arns, S. , Davis, T. L. , & Young, R. N. . (2014). Development of fluorescent substrates and assays for the key autophagy-related cysteine protease enzyme, atg4b. Assay and Drug Development Technologies, 12(3), 176-189.

2. Hennig, A. , Bakirci, Hüseyin, & Nau, W. M. . (2007). Label-free continuous enzyme assays with macrocycle-fluorescent dye complexes. NATURE METHODS, 4(8), 629-632.