DNA staining is used primarily for cell cycle profiling or as one method for measuring apoptosis.
DNA is an important material in the inheritance of life, the quantitative analysis and specific recognition of DNA molecules is of great significance to the development of genomics, virology, molecular biology, and other related disciplines are of great significance. Because of the weak fluorescence of biomolecules, the fluorescence probe method is often used to detect biomolecules. Fluorescence probe method is faster than traditional isotope detection, good repeatability, less sample size, no radiation, so it is widely used in automatic sequencing, antibody immunoassay, disease diagnosis and analysis of anticancer drugs. The sensitivity of DNA fluorescence probe is an important factor affecting the detection results, so it has become the hot point of current research to develop a more sensitive fluorescence probe and avoid the interference of bioluminescence background.
Below is a list of our available Fluorescence probe for DNA (include but not limited to the following):
Ethidium bromide is probably the most famous dye used to display DNA. It can be used in gel mixture, electrophoresis buffer, or staining gel after gel running. The dye molecules are attached to the DNA chain and emit fluorescence under ultraviolet light to accurately show the location of the bands in the gel. Although ethidium bromide has its advantages, its disadvantage is that it is a potential carcinogen and must be dealt with very carefully.
SYBR Gold dye can be used in double or single chain dyeing. As one of the first alternatives to ethidium bromide, SYBR Gold is considered to be more sensitive. Once the dye binds to nucleic acid, it exhibits 1000-fold stronger ultraviolet fluorescence. It can then penetrate thick and high content agarose gel and can be used in formaldehyde gel.
SYBR Green I and II staining were optimized for different uses. Because they bind to DNA, they are still considered potential mutagens, so they should be handled with care. SYBR Green I is more sensitive to double-stranded DNA, while SYBR Green II is most suitable for single-stranded DNA or RNA. Like the popular ethidium bromide dyeing, these highly sensitive dyes can also emit fluorescence under ultraviolet light.
EVA Green is a green fluorescent dye, which has a much less inhibitory effect on Polymerase Chain Reaction (PCR) than other dyes. This makes it very useful for applications such as quantitative real-time PCR. This is also a good choice if you are using a low melting point gel for DNA recovery. It is very stable at high temperature, its own fluorescence is very low, but when combined with DNA, it is high fluorescence.
At present, the problem of DNA fluorescence probe is to improve the sensitivity, enhance the photostability and reduce the synthesis cost. Different DNA fluorescent probes have their own advantages and disadvantages. Near infrared dyes in organic dyes have certain advantages, especially near infrared stomach dyes will be more synthesized and used in the detection of biomolecules. Its photostability needs to be improved. As a new field, quantum dot probe will be paid more and more attention. We provide DNA Stains products and services to support your projects, such as new chemical notification research or drug development requirements.
1. Shalon, D., Smith, S. J., & Brown, P. O. (1996). A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization. Genome research, 6(7), 639-645.
2. Jones, A. C., & Neely, R. K. (2015). 2-aminopurine as a fluorescent probe of DNA conformation and the DNA–enzyme interface. Quarterly reviews of biophysics, 48(2), 244-279.