BODIPY

BODIPY 530/550 C3
BODIPY 530/550 IA
BODIPY 530/550 SE
BODIPY 558/568 C3
BODIPY 558/568 SE
BODIPY 576/589

Background


Dipyrromethene Boron Difluoride (BODIPY) fluorescent substances are a class of emerging dyes with high fluorescence quantum yield, high molar extinction coefficient, good light stability, and insensitivity to pH. By modifying the BODIPY central skeleton structure, a series of derivatives can be obtained and used as a fluorescent probe, a fluorescent label, a photosensitizer, etc. in environmental monitoring and life sciences.

BODIPY

Advantages of BODIPY:

  • Higher molar extinction coefficient, which is beneficial to improve the photosensitivity of dyes and facilitate bioanalysis.
  • Higher fluorescence quantum yield, it can reach 0.6 or more.
  • Narrow fluorescence peak width, it has high detection sensitivity in the field of analysis.
  • Stable spectral properties. Not sensitive to pH, fluorescent probes can be implemented in different solubility, suitable for labeling NDA or biomolecules.
  • Higher photothermal and chemical stability.
  • Smaller molecular weight and lower cytotoxicity.

Below is a list of our available Fluorescence probe for BODIPY (include but not limited to the following):

  • PH fluorescent molecular probe

pH probes generally contain readily dissociable chemical groups, such as amino groups, hydroxyl groups, carboxyl groups, etc., and are detected by protonation/deprotonation resulting in changes in fluorescence. BODIPY derivatives can be used for fluorescence imaging of the acidic nucleus of tumor cells. Under neutral or basic conditions, the lone pair of electrons on the nitrogen atom is transferred to the BODIPY central skeleton structure by photoinduced electronic mechanism (PET), and the compound does not show fluorescence (φ < 0.001). Under acidic conditions, the PET mechanism is blocked by protonation, and the fluorescence of the compound is enhanced.

  • Biomarker field

The BODIPY dye molecule can be covalently bonded to the triphosphate nucleoside analog. The fluorescently labeled G protein can be achieved by the selective recognition of the G protein by the triphosphate nucleoside analog. BODIPY dye molecular marker antibody protein-peptide can be used in biological experiments.

  • Metal ion detection

BODIPY functionalized gold nanoparticles are used for the detection of intracellular copper ions. Using 2,2'-bipyridyl as the recognition group for zinc ions, two BODIPY fluorophores were attached to two pyridine rings. When the fluorescent probe interacts with zinc ions to form a complex, the fluorescence is quenched, while the addition of other ions does not quench the fluorescence.

  • Study on the direction of the tumor as a photosensitizer

Photodynamic therapy (PDT) is an emerging tumor therapy. Currently, porphyrin derivatives are the most commonly used PDT reagents in clinical practice, but their photosensitivity and selectivity are not strong enough, and they are not ideal PDT reagents. BODIPY fluorescent dyes have good photophysical properties and can be used as photodynamic therapy as a class of highly effective photosensitizers as long as they are properly functionalized.

  • Molecular probe

As a novel intracellular messenger molecule, NO is widely involved in the physiological and pathological regulation of the cardiovascular system, nervous system and immune system of the body, and plays an important role in the organism. The novel NO fluorescent probe (TMDABODIPY) rapidly forms a benzotriazole structure when NO is present, and the fluorescence is significantly reduced. The detection limit is 2.5nM, which is an "ON-OFF" type probe.

If you have any BODIPY requirement in mind, please do not hesitate to contact us. We will endeavor to provide highly satisfying products and services!

References

1. Son, H., Lee, H. Y., Lim, J. M., Prof.? Dr. Dongmin Kang, Han, W. S. , & Prof.?Dr. Shim Sung Lee, et al. (2010). A highly sensitive and selective turn-on fluorogenic and chromogenic sensor based on bodipy-functionalized magnetic nanoparticles for detecting lead in living cells. Chemistry, 16(38), 11549-11553.

2. Lee, H. Y., Son, H., Lim, J. M., Oh, J., Kang, D., & Han, W. S., et al. (2010). Bodipy-functionalized gold nanoparticles as a selective fluoro-chromogenic chemosensor for imaging cu2+ in living cells. Analyst, 135(8), 2022-2027.