Sulforhodamine B acid chloride - CAS 62796-29-6
Molecular Formula:
C27H29ClN2O6S2
Molecular Weight:
577.71
COA:
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Application\Fluorophore:
Other Fluorescent Probes
Description:
Sulforhodamine B acid chloride is an amine-reactive red-fluorescent probe for proteins and peptides.
Appearance:
Solid Powder
Synonyms:
Lissamine rhodamine B sulfonylchloride
MSDS:
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Excitation:
568 nm
Emission:
583 nm
InChIKey:
YERWMQJEYUIJBO-UHFFFAOYSA-N
InChI:
InChI=1S/C27H29ClN2O6S2/c1-5-29(6-2)18-9-12-21-24(15-18)36-25-16-19(30(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(28,31)32)17-26(23)38(33,34)35/h9-17H,5-8H2,1-4H3
Canonical SMILES:
CCN(CC)C1=CC2=C(C=C1)C(=C3C=CC(=[N+](CC)CC)C=C3O2)C4=C(C=C(C=C4)S(=O)(=O)Cl)S(=O)(=O)[O-]
1.Texas Res-X and rhodamine Red-X, new derivatives of sulforhodamine 101 and lissamine rhodamine B with improved labeling and fluorescence properties.
Lefevre C1, Kang HC, Haugland RP, Malekzadeh N, Arttamangkul S, Haugland RP. Bioconjug Chem. 1996 Jul-Aug;7(4):482-9.
Texas Red sulfonyl chloride (TR-SC) and Lissamine rhodamine B sulfonyl chloride (LRB-SC) are popular dyes often used to prepare red fluorescent conjugates that are useful second labels in combination with fluorescein. Unfortunately, being sulfonyl chloride derivatives, both are unstable to moisture during storage and prone to hydrolysis in the conjugation reaction. Their instability causes the percentage of reactive dye to vary from lot to lot and requires use of low temperatures and a relatively high pH to optimize conjugation efficiency. Succinimidyl esters of the aminohexanoic acid sulfonamides of both dyes have been prepared, which are designated Texas Red-X succinimidyl ester (TR-X-SE) and Rhodamine Red-X succinimidyl ester, respectively. Their spectral properties are similar to those of their sulfonyl chloride analogs; moreover, incorporation of the succinimidyl ester at the end of the aliphatic chain spacer facilitates conjugation, decreases precipitation of proteins during conjugation and storage, and usually increases the fluorescence yield of the conjugate.
2.Adsorption of acid dye onto organobentonite.
Baskaralingam P1, Pulikesi M, Elango D, Ramamurthi V, Sivanesan S. J Hazard Mater. 2006 Feb 6;128(2-3):138-44. Epub 2005 Dec 19.
Removal of Acid Red 151 from aqueous solution at different dye concentrations, adsorbent doses and pH has been studied. The bentonite clay has been modified using cationic surfactants, which has been confirmed using XRD and FT-IR analyses. Experimental result has shown that the acidic pH favours the adsorption. The adsorption isotherms are described by means of Langmuir and Freundlich isotherms. The adsorption capacity has been found to be 357.14 and 416.66 mg g(-1) for the cetyldimethylbenzylammonium chloride-bentonite (CDBA-bent) and cetylpyridinium chloride-bentonite (CP-bent), respectively. Kinetic studies show that the adsorption followed second-order kinetics.
3.[Adsorption of Acid Red 3R on chitosan and its enhancement by Cu2+ co-sorption].
Li KB1, Zhang T, Wei H, Chen JT, Liu F. Huan Jing Ke Xue. 2009 Sep 15;30(9):2586-91.
Chitosan was used for the removal of Acid Red 3R from aqueous solutions. Batch adsorption studies were carried out to investigate adsorption kinetics and thermodynamics and the effect of coexisting pH, NaCl, and Cu2+ on adsorption. Experimental data were exploited for kinetic and thermodynamic evaluations related to the adsorption processes. The kinetic data correlated well with the pseudo-second-order kinetic model, indicating the chemical sorption via complex formation/ion exchange. The equilibrium data were well fitted by three isotherm models, namely, Langmuir, Freundlich and Dubinin-Radushkevich (D-R) equations. Moreover, adsorption of Acid Red 3R onto the chitosan was found to be strongly depending on solution temperature and pH. However, the addition of sodium chloride was found to have little effect on the adsorption process. Thermodynamic studies revealed the adsorption process was exothermic in natural. The adsorption free energies derived from D-R equation were in range of 9.
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Chemical Structure

CAS 62796-29-6 Sulforhodamine B acid chloride

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