1. Sodium houttuyfonate, a potential phytoanticipin derivative of antibacterial agent, inhibits bacterial attachment and pyocyanine secretion of Pseudomonas aeruginosa by attenuating ﬂagella-mediated swimming motility
Jing Shao • Huijuan Cheng • Changzhong Wang • Daqiang Wu • Xiaoli Zhu • Lingling Zhu • Zhenxin Sun. World J Microbiol Biotechnol (2013) 29:2373–2378
To suppress the bioﬁlmgrowth, drugs used should be able to target the bacterial attachment at the very beginning. However, more P. aeruginosa strains are resistant to traditional antibiotics, and two combined agents have to be adopted to counter the tolerance with more toxicity to the host (Wang et al. 2012). Contrarily, natural phytoanticipin and its derivatives are promising alternatives for bioﬁlm treatment, as they have the advantages of not only lower toxicity and broad-spectrum antibacterial activities, but also lower drug tolerance for a relatively long-time administration (Zeng et al. 2011). Sodium houttuyfonate (SH) is one of derivatives from houttuynin which is a phytoanticipin of Houttuynia cordata Thunb (Saururaceae family) with effective antimicrobial activity (Liu et al. 2011). According to our previous study, Sodium houttuyfonate (SH) alone has a mild suppression on P. aeruginosa bioﬁlm formation with unidentiﬁed mechanism (Shao et al. 2012). However, there have been no reports on antibacterial mechanism of Sodium houttuyfonate (SH) on P. aeruginosa. In this paper, therefore, we mainly focus on the inhibition of SH on bacterial attachment by direct observation and measurement of morphological changes, qRT-PCR analysis of ﬂiC involved in ﬂagella-mediated swimming motility, evaluation of attachment and secreted pyocyanin.