1.Trimethylammonium-Derived Molecular Tweezers and Their Host-Guest Complexation Behaviours in Polar Media.
Gao Z1, Han Y2, Chen J3, Wang X4, Wang F5. Chem Asian J. 2016 Apr 28. doi: 10.1002/asia.201600364. [Epub ahead of print]
Trimethylammonium-derived alkynylplatinum(II) terpyridine molecular tweezers have been designed and synthesized in this manuscript. They show moderate binding affinity towards the complementary organoplatinum guest in polar media such as DMSO, methanol and water, even though the guest itself is sparingly soluble in the latter two solvents. Responsive guest encapsulation/release from the cavity of molecular tweezers can be achieved via successive addition and removal of trifluoroacetic acid, accompanying with remarkable switching of the photo-physical signals, which would benefit for the fabrication of intelligent supramolecular materials with tailored functionalities.
2.Transgenic Expression of Human Lysophosphatidic Acid Receptor LPA2 in Mouse Intestinal Epithelial Cells Induces Intestinal Dysplasia.
Yoshida M1,2, He P1, Yun CC1,3. PLoS One. 2016 Apr 28;11(4):e0154527. doi: 10.1371/journal.pone.0154527.
Lysophosphatidic acid (LPA) acts on LPA2 receptor to mediate multiple pathological effects that are associated with tumorigenesis. The absence of LPA2 attenuates tumor progression in rodent models of colorectal cancer, but whether overexpression of LPA2 alone can lead to malignant transformation in the intestinal tract has not been studied. In this study, we expressed human LPA2 in intestinal epithelial cells (IECs) under control of the villin promoter. Less than 4% of F1-generation mice had germline transmission of transgenic (TG) human LPA2; as such only 3 F1 mice out of 72 genotyped had TG expression. These TG mice appeared anemic with hematochezia and died shortly after birth. TG mice were smaller in size compared with the wild type mouse of the same age and sex. Morphological analysis showed that TG LPA2 colon had hyper-proliferation of IECs resulting in increased colonic crypt depth. Surprisingly, TG small intestine had villus blunting and decreased IEC proliferation and dysplasia.
3.Sensitivity of HIV rapid tests compared to fourth generation enzyme immunoassays or HIV RNA tests - a systematic review and meta-analysis.
Tan WS1, Chow EP, Fairley CK, Chen MY, Bradshaw CS, Read TR. AIDS. 2016 Apr 27. [Epub ahead of print]
OBJECTIVE: Determine the sensitivity of rapid HIV tests (RT) compared to fourth generation enzyme immunoassays (EIA) or nucleic acid amplification tests (NAAT) in clinical settings.
4.Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice.
Yin G1, Whelan J2, Wu S1, Zhou J1, Chen B1,3, Chen X1, Zhang J1, He J1, Xin X1, Lu X1. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013.
The critical node (CN) in seed aging in rice (Oryza sativa) is the transformation from Phase I (P-I) to Phase II (P-II) of the reverse S-shaped curve (RS-SC). Although mitochondrial dysfunction plays a key role in seed ageing, the metabolic shift in the CN remains poorly understood. Here, we investigated the mitochondrial regulatory mechanisms during the CN of rice seed ageing. We showed that during the CN of seed ageing, the mitochondrial ultrastructure was impaired, causing oxygen consumption to decrease, along with cytochrome c (cyt c) oxidase and malate dehydrogenase (MDH) activity. In addition, the transcript levels for the alternative pathway of the electron transport chain (ETC) were significantly induced, whereas the transcripts of the cytochrome oxidase (COX) pathway were inhibited. These changes were concomitant with the down-regulation of mitochondrial protein levels related to carbon and nitrogen metabolism, ATP synthase (ATPase) complex, tricarboxylic acid cycle (TCA) cycle, mitochondrial oxidative enzymes, and a variety of other proteins.