Pyronin Y - CAS 92-32-0
Molecular Formula:
Molecular Weight:
DNA Stains
Pyronin Y is a fluorescent probe that stains double stranded RNA in living or fixed cells as well as in tissues. It has been shown to accumulate in the mitochondria of viable cells.
Solid Powder
Pyronine Y; 3,6-bis(dimethylamino)-xanthylium, monochloride
Soluble in DMSO.
Store at -20°C
540-550 nm
560-580 nm
Canonical SMILES:
1.Ultrastructural features of Beta leaves infected with beet yellows virus.
Cronshaw J;Hoefert L;Esau K J Cell Biol. 1966 Dec;31(3):429-43.
A cytochemical and electron microscope study has been made of leaves of sugar beet infected with beet yellows virus. Inclusions of particles, which agree in size with beet yellows virus particles isolated by other investigators, have been localized in the ground cytoplasm, in the chloroplasts, and in the nuclei. These particles are circa 100 A in diameter and have an electron-transparent core of 30 to 40 A. Use of acridine orange, azure B, and pyronine Y has revealed that the cytoplasmic inclusion bodies, which consist wholly of the elongate particles, have a strong RNA reaction removable by RNase pretreatment. Particles observed in the chloroplasts may or may not be associated with lipid spheres. If they are, the particles are confined to the periphery of the spheres. In this position the particles are arranged tangentially and are further arranged parallel into groups which lie at various angles to one another. Within the groups the particles are regularly spaced in a three dimensional lattice. Particles located free in the stromal regions are often arranged regularly in curved rows which lie parallel to one another so that a three dimensional lattice is formed. The dispersed and compact forms of virus inclusions are described and related to the condition of the associated cytoplasm.
2.Localization of a nonintercalative DNA binding antitumour drug in mitochondria: relationship to multidrug resistance.
Liley DT;Wiggins PM;Baguley BC Eur J Cancer Clin Oncol. 1989 Sep;25(9):1287-93.
The bis-(n-butyl) quaternary salt of N,N'-bis-(6-quinolyl)terephthalamide (QBQ), a fluorescent antitumour compound in the phthalanilide series which is thought to bind to the minor groove of the DNA double helix, has been investigated with respect to its in vitro activity and subcellular localization. Cultured MCF-7 human breast carcinoma cells concentrated QBQ in mitochondria by a time-dependent process which was inhibited by the ionophore valinomycin, suggesting a possible mode of antitumour action of QBQ through mitochondrial poisoning. Growth of cultured P388 murine leukaemia cells was inhibited 50% in the presence of 0.52 microM QBQ and multidrug-resistant P388 sublines developed for resistance to actinomycin D, vincristine, Adriamycin and the phthalanilide NSC 38280 were cross-resistant to the drug. Cross-resistance was reduced in all lines by the presence of 11 microM verapamil, suggesting that a transport resistance mechanism operates on QBQ. The actinomycin D-resistant P388 cell line was found to be cross-resistant to the aromatic cations rhodamine 123, which binds to proteins, and ethidium and pyronin Y, which bind intercalatively to DNA. Thus mitochondrion-specific drugs with different macromolecular binding properties all appear to be excluded by multidrug-resistant cells.
3.Dual optoelectronic visual detection and quantification of spectroscopically silent heavy metal toxins: a multi-measurand sensing strategy based on Rhodamine 6G as chromo or fluoro ionophore.
Prathish KP;James D;Jaisy J;Prasada Rao T Anal Chim Acta. 2009 Aug 4;647(1):84-9. doi: 10.1016/j.aca.2009.04.044. Epub 2009 May 5.
A novel colorimetric chemo-sensor for the simultaneous visual detection and quantification of spectroscopically silent heavy metal toxins viz. cadmium, lead and mercury has been developed. This is based on the proposed sequential ligand exchange (SLE) mechanism of iodide from Pb-I(-)-Rhodamine 6G ion associate with citrate (without affecting ion associates of Cd and Hg) and subsequently from Cd-I(-)-Rhodamine 6G ion associate with EDTA (without affecting Hg-I(-)-Rhodamine 6G). Multi-measurand detection and quantification by colorimetry is possible as the individual toxins gives identical bathochromic shifts in aqueous solution, i.e. from 530 to 575 nm on formation of ternary ion associates in singular, binary and ternary mixtures. The visual detection provides a simple, quick and sensitive detection method in addition to quantification via spectrophotometry with Sandell sensitivities of 1.1, 15 and 2.5 microg dm(-2) for cadmium, lead and mercury, respectively. The developed procedure has been successfully tested for the analysis of environmental (cast alkali, lead acid battery and zinc manufacturing industry effluents) samples. Furthermore, the multi-measurand quantification of the above-mentioned heavy metal toxins based on fluorescence quenching and use of Pyronine G as chromo-ionophore instead of Rhodamine 6G is also described.
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CAS 92-32-0 Pyronin Y

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