|Description||Nuclear yellow, as Hoechst S769121, belongs Hoechst stains, which were originally developed by Hoechst AG, which numbered all their compounds and are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. It is the long-wavelength tracer and is often combined with the popular retrograde tracer true blue for two-color neuronal mapping. It exhibits excitation/emission maxima ~335/495 nm when bound to DNA. In neuronal cells, it primarily stains the nucleus with yellow fluorescence. It is stable when subjected to immunohistochemical processing and can be used to photoconvert DAB into an insoluble, electron-dense reaction product.|
|Appearance||Light yellow powder|
|Synonyms||4-[6-[6-(4-Methyl-1-Piperazinyl)-1H-Benzimidazol-2-Yl]-1H-Benzimidazol-2-Yl]Benzenesulfonamide Trihydrochloride;4-(5-(4-Methyl-1-Piperazinyl)(2,5'-Bi-1H-Benzimidazol)-2'-Yl)Benzenesulfonamide Trihydrochloride;Hoe-S769121;2-(4-Sulfamylphenyl)-6-[6-(4-methylpiperazino)-2-benzimidazolyl]benzimidazole trihydrochloride|
|Solubility||H2O: ≥ 6 mg/mL, DMSO: ＜ 12.2 mg/mL|
|Storage||-20°C Freezer, Protect from light|
|Application||Nuclear yellow exhibits excitation/emission maxima ~335/495 nm when bound to DNA. In neuronal cells, it primarily stains the nucleus with yellow fluorescence.|
|Quality Standard||In-house standard|
|Shelf Life||2 month in rt, long time|
|Boiling Point||813.6°C at 760 mmHg|
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