We operate as professional lipid nanoparticle (LNP) service providers with a commitment to provide complete protein delivery solutions. Our experienced R&D team utilizes our advanced technology platform to develop high-performance lipid nanoparticles that meet your specific requirements enabling both efficient and effective protein delivery. We deliver comprehensive solutions that cover everything from protein analysis and modification to customized LNP formulation as well as protein encapsulation and purification alongside thorough functional validation. The delivery system achieves stability through these integrated steps which also ensure precise targeting and biological activity. Our reliable, high-quality services support both fundamental research projects and therapeutic product development efforts to enable successful breakthrough results.
Lipid nanoparticles facilitate cellular uptake through interactions with the cell membrane and endocytic pathways, releasing proteins intracellularly. This enables targeted delivery to specific cells or tissues, significantly enhancing bioavailability and therapeutic effectiveness.
The bilayer structure of LNPs encapsulates proteins, shielding them from the external environment. This protects proteins from enzymatic degradation in vivo, prolongs their half-life, and enhances overall stability.
By modifying and encapsulating proteins within LNPs, immune recognition and clearance are minimized, lowering immunogenic responses and improving safety and tolerability.
Surface modification of LNPs with targeting ligands such as antibodies, peptides, or other molecules allows selective binding to target cells or tissues, improving therapeutic precision and minimizing off-target effects.
The lipid composition and nanoparticle architecture can be fine-tuned to regulate the rate and duration of protein release, enabling sustained or stimulus-responsive delivery for prolonged therapeutic benefit.
LNP components are generally biocompatible, allowing for gradual metabolism and clearance without long-term toxicity, ensuring a high safety profile.
Ionizable Lipid Selection & Synthesis: We help select the optimal ionizable lipids from our extensive catalog or synthesize novel lipids tailored to your project to maximize LNP performance.
LNP Formulation & Optimization: Our end-to-end formulation services customize LNPs with controlled particle sizes and compositions. Utilizing advanced high-throughput screening, biophysical characterization, and functional assays, we optimize lipid ratios, particle charge, size, and encapsulation efficiency to deliver the safest and most effective formulations.
Preformulation Studies: We characterize the physicochemical properties of your protein therapeutics to guide the rational design of LNPs that preserve protein stability and efficacy.
In Vitro Cell Transfection: We assess the cellular uptake, intracellular trafficking, and functional impact of LNP-encapsulated proteins in relevant cell culture models.
In Vivo Delivery: Using animal models, we evaluate biodistribution, targeting specificity, bioavailability, and pharmacodynamics to ensure robust efficacy and safety.
Physicochemical Characterization: We conduct comprehensive analyses including particle size and distribution (DLS), zeta potential, and morphology (TEM) to verify LNP quality and uniformity.
Encapsulation Efficiency & Loading Capacity: Through HPLC, UV-Vis spectrophotometry, and other analytical methods, we accurately quantify protein loading and encapsulation efficiency.
Stability Testing: Our stability assessments cover various environmental conditions, including temperature, pH, and storage time, using accelerated and long-term studies to predict shelf life and optimize handling protocols.
Targeted Delivery System Development: We design targeted LNPs for specific cell types-such as tumor cells, hepatocytes, or neurons-by decorating nanoparticle surfaces with targeting ligands like antibodies, peptides, or glycans, enhancing delivery precision and therapeutic impact.
Multifunctional Delivery Systems: Addressing advanced therapeutic needs, we develop multifunctional LNPs capable of co-delivering proteins with nucleic acids or small molecules to enable synergistic effects, beneficial in gene editing, immunotherapy, and beyond.
Protein Modification: We provide protein modifications (e.g., PEGylation, glycosylation) to enhance protein stability, extend circulation half-life, and improve encapsulation efficiency.
Analytical Method Development and Validation: We develop and validate customized analytical methods, including HPLC and mass spectrometry, for rigorous quality control and pharmacokinetic studies of LNP formulations, ensuring precise and dependable results.
Conduct thorough analysis of target proteins to understand their structure and function. Perform modifications to enhance protein stability, extend half-life, reduce immunogenicity, and add targeting sequences for precise delivery.
Choose appropriate lipid components (cationic lipids, helper lipids, cholesterol, PEGylated lipids) based on protein properties and delivery requirements. Prepare LNPs using methods like ethanol injection, thin-film hydration, or microfluidics to ensure uniform particle size and stable structure.
Mix modified proteins with LNPs using electrostatic interactions, hydrophobic forces, or covalent bonding to encapsulate or bind proteins on/inside LNPs. Optimize protein-to-lipid ratio and mixing conditions to maximize loading efficiency and particle stability.
Purify loaded LNPs to remove free protein and impurities using centrifugation, dialysis, or ultrafiltration. Characterize physical-chemical properties (particle size/distribution via DLS, surface charge via zeta potential) and quantify protein loading using HPLC or UV-Vis spectroscopy.
Evaluate delivery efficiency, protein release profile, and biological activity in vitro (cell culture) and in vivo (animal models). Based on results, fine-tune lipid composition, manufacturing process, and protein modifications to improve overall delivery performance and meet application-specific goals.
Advanced Analytical & Manufacturing CapabilitiesEquipped with state-of-the-art instruments such as dynamic light scattering (DLS), zeta potential analyzers, and high-performance liquid chromatography (HPLC) to provide accurate, reliable data and consistent LNP quality.
Experienced, Multidisciplinary R&D TeamComprised of experts in pharmaceutical sciences, biology, chemistry, and materials science with PhD-level training and rich research experience, ensuring professional design and problem-solving.
Highly Customizable ServiceFlexible design and development tailored to unique protein characteristics and client requirements-from small-scale research projects to large-scale preclinical studies-ensuring optimal delivery solutions.
Comprehensive One-Stop PlatformEnd-to-end services including protein analysis, LNP formulation, protein loading, purification, characterization, and functional testing, simplifying project management, reducing turnaround time, and ensuring strict quality control throughout all stages.
In theory, a wide range of proteins-including soluble and membrane proteins-can be delivered using LNPs. However, the actual performance depends on the protein's properties, structure, and its interaction with the nanoparticles. Some proteins may require specific modifications or optimizations to improve delivery efficiency.
Delivery efficiency varies due to multiple factors, including the protein's properties (e.g., size, charge), LNP composition and structure (e.g., lipid type, particle size, surface charge), cell type, and delivery conditions. Generally, optimizing the LNP formulation and preparation process, along with appropriate protein modifications, can significantly enhance delivery efficiency.
