In the cutting-edge fields of RNA therapy and vaccine development, RNA delivery technology is a crucial factor that determines success or failure. Our lipid nanoparticle (LNP) RNA delivery service offers a comprehensive and professional solution for researchers and companies, covering the entire process from formulation design, RNA loading, product testing to delivery efficacy evaluation. We are dedicated to improving RNA delivery efficiency, enhancing stability, and achieving precise targeting, fully supporting your RNA projects from the laboratory to clinical applications.
Based on your RNA type (e.g., mRNA, siRNA) and target cell characteristics, as well as the expected delivery outcomes, our professional team will utilize extensive lipid nanoparticle technology knowledge to tailor LNP formulations. We carefully select appropriate ionizable lipids to ensure LNPs can form stable complexes with RNA in physiological environments and effectively release RNA inside target cells. Additionally, we optimize the LNP structure and performance by incorporating neutral helper lipids (such as cholesterol, DOPE, etc.), improving delivery efficiency and biocompatibility. Moreover, we can functionalize LNPs by adding targeting ligands or fluorescent markers to meet specific research or application needs.
Using advanced preparation techniques, we efficiently load RNA into LNPs. We optimize the RNA-to-LNP mixing ratio and reaction conditions (such as temperature, pH, and ionic strength) to maximize RNA encapsulation, while ensuring RNA integrity and biological activity are not affected. Our preparation process also effectively controls LNP particle size distribution, keeping it within an optimal range for subsequent delivery and application.
Equipped with advanced detection devices and technologies, we perform comprehensive characterization and analysis of the prepared LNPs. We can accurately measure LNP particle size distribution, zeta potential, and ensure uniform particle size and stable charge, which are critical for LNP distribution and delivery efficiency in vivo. Additionally, we can measure RNA encapsulation efficiency and assess RNA loading in LNPs, ensuring batch-to-batch quality consistency. We also analyze LNP morphology and structure, using techniques such as transmission electron microscopy (TEM) to further verify LNP quality and characteristics.
We comprehensively evaluate the delivery efficiency of LNP-RNA complexes in both in vitro cell models and in vivo animal models. In vitro testing uses various cell lines to simulate different physiological environments, assessing RNA cell uptake efficiency, intracellular distribution, and gene expression levels to evaluate LNP delivery performance and cell compatibility. In vivo testing involves selecting suitable animal models and using different administration routes (such as intravenous or intramuscular injection) to observe the distribution, pharmacokinetics, and biological effects of LNP-RNA complexes in the body, including RNA expression levels in target tissues, therapeutic effects, and potential side effects. We provide detailed experimental data to support your project decision-making and progress.
You will discuss your project needs with our professional team, including RNA type, target cells, and expected delivery outcomes. We will evaluate the feasibility of the project and create an initial service plan tailored to your needs.
Our R&D team will begin designing the LNP formulation, selecting the best lipid combination and ratio through multiple rounds of experiments to achieve efficient RNA delivery.
Your RNA will be loaded into the optimized LNPs using advanced preparation techniques, ensuring efficient encapsulation while maintaining RNA biological activity.
We will perform comprehensive testing of the LNP-RNA complex, including particle size distribution, zeta potential, encapsulation efficiency, and RNA integrity, to ensure consistent and reliable product quality.
We will assess delivery efficacy in both in vitro cell models and in vivo animal models, measuring RNA uptake efficiency, gene expression levels, and the achievement of biological functions, providing you with detailed experimental reports.
This platform utilizes microfluidic technology to precisely control the preparation process of lipid nanoparticles, combined with liposome technology to optimize the composition and performance of the particles, ensuring efficient and stable RNA delivery.
This platform synthesizes high-quality mRNA through in vitro transcription technology and is capable of synthesizing other types of nucleic acid molecules, providing a diverse molecular basis for RNA delivery.
This platform uses physicochemical characterization, biological assessments, and safety evaluations to comprehensively test the quality and performance of lipid nanoparticles, ensuring their safety and effectiveness during the delivery process.
This platform focuses on evaluating the delivery efficiency of lipid nanoparticles in in vitro cell models and in vivo animal models, optimizing the particles based on the evaluation results to enhance their targeting ability and delivery efficiency to specific tissues or cells.
Professional TeamOur team brings together senior experts in lipid nanoparticle technology and RNA biology, possessing rich R&D experience and deep professional knowledge to provide precise technical support for your projects.
Customized ServicesWe fully understand the uniqueness of each project and offer a comprehensive range of customized services, from formulation design to efficacy evaluation, tailored to meet your specific needs.
Strict Quality ControlWe have established rigorous quality control standards at every stage of the service process, from raw material selection to finished product testing, ensuring that the LNP-RNA delivery products we deliver have excellent performance and stable quality.
Fast DeliveryWith optimized service processes and an efficient experimental team, we can complete your project in the shortest time possible, accelerating your research and development progress.
Infectious Disease Vaccines: The successful application of the COVID-19 mRNA vaccine has demonstrated the potential of LNP technology in rapidly developing efficient vaccines. In addition to COVID-19, LNP delivery systems can be used to develop mRNA vaccines for other infectious diseases such as influenza, HIV, and Ebola.
Cancer Vaccines: LNPs can deliver mRNA encoding tumor-associated antigens, stimulating the immune system to recognize and attack tumor cells. For example, companies like BioNTech are developing mRNA vaccines for various cancers.
Immunotherapy: LNP-delivered RNA can encode immune-modulatory proteins or cytokines, enhancing the body’s immune response to tumors. Additionally, siRNA can be delivered to silence immune-suppressing genes in tumor cells, thereby enhancing immunotherapy effects.
Gene Therapy: LNPs can deliver gene-editing tools such as CRISPR/Cas9 to edit pathogenic genes in tumor cells, correcting gene mutations or regulating gene expression to treat cancer.
Gene Editing: For genetic diseases caused by single-gene mutations, such as cystic fibrosis and sickle cell anemia, LNPs can deliver gene editing tools to repair or replace the pathogenic gene in patients’ bodies.
Protein Replacement Therapy: In certain genetic diseases where patients’ cells cannot produce specific proteins, LNP-delivered mRNA can guide the synthesis of the missing protein within the cells, alleviating disease symptoms.
Antiviral Therapy: Apart from vaccine development, LNPs can deliver siRNA or antisense oligonucleotides to specifically target viral genes and inhibit viral replication, which can be used for treating viral infections.
Antibacterial Therapy: For antibiotic-resistant bacterial infections, LNP-delivered RNA can encode antimicrobial peptides or proteins that regulate bacterial metabolism, enhancing the body’s resistance to bacteria.
Tissue Repair and Regeneration: LNP-delivered RNA can encode growth factors or cytokines that promote tissue repair and regeneration, used in the treatment of myocardial infarction, nerve damage, osteoarthritis, and other diseases.
Stem Cell Therapy: RNA delivered by LNPs can regulate stem cell differentiation and proliferation, guiding stem cells to differentiate into specific cell types for tissue repair.
Gene Function Research: In basic biological research, LNP-delivered siRNA or mRNA can be used for gene knockdown or overexpression experiments to help researchers study the functions of specific genes.
Drug Screening: The LNP delivery system can be used in high-throughput drug screening, quickly evaluating the effects of drugs on their target by delivering RNA that encodes the drug target.
LNPs provide strong protection for RNA. Their lipid bilayer structure effectively blocks the intrusion of nucleases, preventing RNA degradation. Additionally, the stability of LNPs in physiological environments has been well validated, ensuring that RNA remains intact in the bloodstream, prolonging its in vivo duration of action, and enhancing therapeutic effects.
Yes, we understand that every project has unique requirements. Therefore, we provide a full range of customized LNP services, optimizing and adjusting every step from formulation design to delivery efficacy evaluation to ensure that our services perfectly align with your research goals.
