Our company specializes in lipid nanoparticle (LNP) business and offers detailed professional characterization services for lipid nanoparticles. Our research team uses advanced instruments to deliver precise analyses of key lipid nanoparticle parameters including particle size distribution, zeta potential, morphology, encapsulation efficiency and drug loading capacity. Our detailed data support helps ensure quality control and performance evaluation of your nanoparticle drug delivery systems and new lipid nanoparticle materials which enables your research and development projects to progress smoothly.
Dynamic Light Scattering (DLS): The hydrodynamic diameter of lipid nanoparticles is determined using DLS which identifies the size of an equivalent spherical particle that diffuses at the same rate as the analyzed particle. The method generates a particle size distribution curve from light intensity measurements alongside average particle size and polydispersity index for evaluating size distribution uniformity and concentration.
Nanoparticle Tracking Analysis (NTA): The Nanoparticle Tracking Analysis (NTA) technique evaluates individual lipid nanoparticle dimensions and concentrations while presenting visual data about size distribution patterns to better understand size variance in samples.
Laser Diffraction (LD): The technique uses light scattering to determine particle size distribution and works effectively for lipid nanoparticles that display a wide range of sizes.
Electrophoretic Light Scattering (ELS): Used to determine the Zeta potential of lipid nanoparticles, which represents the electric potential on the sliding plane of particles in a liquid medium. This can reflect the surface charge and electrostatic repulsion or attraction between particles, providing insights into the colloidal stability and aggregation behavior of lipid nanoparticles.
Transmission Electron Microscopy (TEM): Allows direct observation of the morphology, structure, and particle size of lipid nanoparticles, providing high-resolution particle images to help understand the particle shape and detect any aggregation.
Cryo-Transmission Electron Microscopy (Cryo-TEM): For lipid nanoparticles that are sensitive to electron beams or prone to dehydration and deformation at room temperature, Cryo-TEM quickly freezes the sample at low temperatures, preserving its original state for observation, offering a more accurate reflection of the true morphology and structure of the particles.
High-Performance Liquid Chromatography (HPLC): Used to determine the drug content encapsulated in lipid nanoparticles, which helps calculate the encapsulation efficiency, i.e., the ratio of the actual amount of encapsulated drug to the theoretical drug dose. It can also analyze drug loading to assess the drug delivery capacity of lipid nanoparticles.
UV-Vis Spectrophotometry: When the drug or lipid itself has characteristic UV or visible light absorption, UV-Vis can be used to measure absorbance, from which the encapsulation efficiency and drug loading can be calculated.
Accelerated Stability Testing: Lipid nanoparticle samples are placed in an environment with a temperature higher than normal storage conditions, and samples are regularly taken to monitor changes in parameters such as particle size, zeta potential, and encapsulation efficiency. This helps predict the sample's stability under normal storage conditions.
Long-Term Stability Monitoring: Lipid nanoparticle samples are tracked and monitored over an extended period under actual storage conditions, with regular testing of various parameters to evaluate their stability throughout the product's shelf life.
Gas Chromatography-Mass Spectrometry (GC-MS): Used to analyze the types and contents of lipids in lipid nanoparticles, which helps to determine whether the component composition meets the required specifications.
Liquid Chromatography-Mass Spectrometry (LC-MS): LC-MS can perform both qualitative and quantitative analysis of drugs or other components encapsulated in lipid nanoparticles, providing detailed information about their composition.
Nucleic Acid Analysis: For lipid nanoparticles loaded with nucleic acids, methods such as fluorescence labeling, nucleic acid dyes, and equipment like nanoparticle flow cytometers can be used to precisely analyze the nucleic acid content, localization, and copy number within the particles, thereby assessing the encapsulation and integrity of the nucleic acids.
Thermal Analysis: Using techniques such as Differential Scanning Calorimetry (DSC), thermal analysis studies the thermal stability, phase transition temperature, and other properties of lipid nanoparticles, providing insights into their behavior under different temperature conditions.
Clients provide details about their lipid nanoparticle, and our technical team offers advice and a proposal based on their needs.
Clients prepare and submit the lipid nanoparticle samples along with a form detailing the sample information, such as name, preparation method, and storage.
After receiving samples, we check their quality and confirm the final service items with the client, followed by signing a service contract.
Our technical team conducts tests using advanced equipment, following strict procedures to ensure data accuracy.
The test data is analyzed using specialized software to assess the performance and quality of the lipid nanoparticles.
The final report is delivered to the client, with prompt communication to address questions and offer technical support.
Expert TeamOur experts have strong academic backgrounds and years of practical experience in lipid nanoparticles, covering materials science, pharmacology, and biology. We can accurately identify key challenges and provide professional, in-depth services.
Advanced EquipmentWe use top-tier instruments like high-precision DLS, high-performance TEM, and high-resolution LC-MS, ensuring accurate, sensitive, and reliable characterization results to support product development and quality control.
Tailored ServicesEvery client's lipid nanoparticles are different. We design customized characterization plans based on your specific needs, choosing the most suitable techniques and methods to accelerate your R&D.
Full-Service CoverageFrom basic particle size, zeta potential, morphology, and component analysis to encapsulation efficiency, drug release studies, and stability evaluation - we offer complete services across all R&D stages, saving you time and resources.
Strict Quality ControlWe have a comprehensive quality control system ensuring every step of the process, from sample preparation to data analysis, follows strict standards, ensuring accurate and reproducible results.
Competitive PricingWe offer high-quality services at affordable rates, ensuring cost-efficiency by optimizing operational costs and resource utilization.
We will provide a detailed characterization report, which includes the experimental objectives, methods, conditions, raw data, data analysis, and conclusions. The report will be available in both electronic (PDF format) and printed versions for easy access and archiving.
We have a professional technical team and advanced instrumentation, strictly following standardized operating procedures and a quality control system to ensure the high accuracy and reliability of the characterization results.
