Lucifer Yellow CH dilithium salt - CAS 67769-47-5
Molecular Formula:
C13H9Li2N5O9S2
Molecular Weight:
457.2
COA:
Inquire
Application\Fluorophore:
Cell and Organelle Stains
Description:
Lucifer yellow CH is a fluorescent probe that stains nerve cells. It is the hydrazine form of Lucifer Yellow VS and contains a free hydrazido group that reacts with aliphatic aldehydes. It has been used as a biological tracer for monitoring the branching pattern and regeneration of neurons, gap junction detection and characterization, and selective ablation of cells following aldehyde fixation.
Purity:
≥80%
Appearance:
Solid Powder
Synonyms:
Lucifer Yellow carbohydrazide; 6-amino-2-[(hydrazinylcarbonyl)amino]-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinoline-5,8-disulfonic acid dilithium salt
Storage:
Store at -20°C
MSDS:
Inquire
Excitation:
430 nm
Emission:
540 nm
InChIKey:
RPKCZJYDUKVMGF-UHFFFAOYSA-L
InChI:
InChI=1S/C13H11N5O9S2.2Li/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2
Canonical SMILES:
[Li+].[Li+].C1=C(C=C2C3=C1C(=O)N(C(=O)C3=CC(=C2N)S(=O)(=O)[O-])NC(=O)NN)S(=O)(=O)[O-]
1.Visualization of neurons filled with biotinylated-lucifer yellow following identification of efferent connectivity with retrograde transport.
Hill SJ1, Oliver DL. J Neurosci Methods. 1993 Jan;46(1):59-68.
A new method is described that allows identification of neurons by retrograde transport, intracellular injection of biotin-labeled Lucifer Yellow, and a histochemical reaction of the biotin. Cells in the inferior colliculus that project to the ipsilateral thalamus are identified by injection of rhodamine-labeled latex beads into the medial geniculate body and retrograde transport in vivo. Several days later, the brains are fixed with aldehydes, and the inferior colliculus is cut on a vibratome. The rhodamine-labeled cells are observed with epi-fluorescent optics, impaled with intracellular pipettes under visual control, and injected with 9% Lucifer Yellow dilithium salt and 1% Lucifer Yellow cadaverine biotin-X. The biotinylated Lucifer Yellow is visualized by incubation of the slice overnight in avidin-biotin-HRP complex in the presence of 0.1% Triton X-100 at 4 degrees C. A diaminobenzidine reaction with simultaneous cobalt and nickel intensification follows.
Molecular Weight Calculator Molarity Calculator Solution Dilution Calculator

Related Cell and Organelle Stains Products


5-JOE, AM diacetate

5-JOE, AM diacetate is a fluorescent probe used for detection of cell viability.

FG 488 DHPE

FG 488 DHPE is a phospholipid fluorescent probe with increased photostability compared to fluorescein.

CAS 77172-72-6 3,4,5,6-Tetrabromophenol sulfonephthalein

3,4,5,6-Tetrabromophenol sulfonephthalei
(CAS: 77172-72-6)

3,4,5,6-Tetrabromophenol sulfonephthalein is a stain for cell biology.

Vybrant DiD Cell-Labeling Solution

Vybrant DiD Cell-Labeling Solution is a dye delivery solution used in flow cytometry and fluorescence microscopy.

CAS 143556-24-5 L 012 sodium salt

L 012 sodium salt
(CAS: 143556-24-5)

L 012 sodium salt is a luminal-based chemiluminescent dye and probe. It is used to detect superoxide in biological systems, specifically in whole inflammatory c...

CAS 67769-47-5 Lucifer Yellow CH dilithium salt

Lucifer Yellow CH dilithium salt
(CAS: 67769-47-5)

Lucifer yellow CH is a fluorescent probe that stains nerve cells. It is the hydrazine form of Lucifer Yellow VS and contains a free hydrazido group that reacts ...

C6 NBD L-threo-ceramide

C6 NBD L-threo-ceramide is a cell-permeable analog of ceramides that is tagged with a fluorescent group C6 nitrobenzoxadiazole (C6 NBD). It is rapidly transferr...

Biotin-X-ethylenediamine

Biotin-X-ethylenediamine is a biotinylation reagent used for labeling DNA, carboxylic acids and other biomolecules. It has been used for neuronal tracing and in...

Chemical Structure

CAS 67769-47-5 Lucifer Yellow CH dilithium salt

Quick Inquiry

Verification code

Featured Items