Lucifer Yellow CH dilithium salt - CAS 67769-47-5
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Cell and Organelle Stains
Lucifer yellow CH is a fluorescent probe that stains nerve cells. It is the hydrazine form of Lucifer Yellow VS and contains a free hydrazido group that reacts with aliphatic aldehydes. It has been used as a biological tracer for monitoring the branching pattern and regeneration of neurons, gap junction detection and characterization, and selective ablation of cells following aldehyde fixation.
Solid Powder
Lucifer Yellow carbohydrazide; 6-amino-2-[(hydrazinylcarbonyl)amino]-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinoline-5,8-disulfonic acid dilithium salt
Store at -20°C
430 nm
540 nm
Canonical SMILES:
1.Visualization of neurons filled with biotinylated-lucifer yellow following identification of efferent connectivity with retrograde transport.
Hill SJ1, Oliver DL. J Neurosci Methods. 1993 Jan;46(1):59-68.
A new method is described that allows identification of neurons by retrograde transport, intracellular injection of biotin-labeled Lucifer Yellow, and a histochemical reaction of the biotin. Cells in the inferior colliculus that project to the ipsilateral thalamus are identified by injection of rhodamine-labeled latex beads into the medial geniculate body and retrograde transport in vivo. Several days later, the brains are fixed with aldehydes, and the inferior colliculus is cut on a vibratome. The rhodamine-labeled cells are observed with epi-fluorescent optics, impaled with intracellular pipettes under visual control, and injected with 9% Lucifer Yellow dilithium salt and 1% Lucifer Yellow cadaverine biotin-X. The biotinylated Lucifer Yellow is visualized by incubation of the slice overnight in avidin-biotin-HRP complex in the presence of 0.1% Triton X-100 at 4 degrees C. A diaminobenzidine reaction with simultaneous cobalt and nickel intensification follows.
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CAS 67769-47-5 Lucifer Yellow CH dilithium salt

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