Ligupurpuroside B - CAS 147396-02-9
Catalog number: 147396-02-9
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anti-lipid peroxidation
Ligupurpuroside B; 3)-, 4-[(2E)-3-(4-hydroxyphenyl)-2-propenoate] (9CI); b-D-Glucopyranoside,2-(4-hydroxyphenyl)ethyl O-6-deoxy-a-L-mannopyranosyl-(1®
anti-lipid peroxidation
1.Antioxidative glycosides from the leaves of Ligustrum robustum.
He ZD;Lau KM;But PP;Jiang RW;Dong H;Ma SC;Fung KP;Ye WC;Sun HD J Nat Prod. 2003 Jun;66(6):851-4.
Phytochemical investigation of the ethanol extract of the leaves of Ligustrum robustum, monitored by a bioassay involving the hemolysis of red blood cells induced by 2,2'-azo-bis(2-amidinopropane) dihydrochloride, led to the isolation of three new glycosides, ligurobustosides M (1), N (2), and O (3), along with 10 known ones, osmanthuside B (4), osmanthuside B6 (5), acteoside (6), ligupurpuroside A (7), ligupurpuroside B (8), ligurobustoside C (9), ligurobustoside E (10), ligurobustoside I (11), cosmosiin (12), and rhoifolin (13). The structures of the new compounds were elucidated by spectroscopic methods. Seven of the glycosides showed stronger antioxidant effects than the standard, trolox.
2.Quantitation of ligupurpurosides B and C in rat plasma using HPLC-MS/MS.
Chen QQ;Guo JR;Feng SM;Wang CY;Zhang W Chin J Nat Med. 2016 Jun;14(6):473-80. doi: 10.1016/S1875-5364(16)30045-0.
The present study was designed to develop a sensitive and selective specific high performance liquid chromatography (HPLC)-tandem mass spectrometric method (MS/MS) for the determination of ligupurpurosides B and C in rat plasma. The samples were prepared after protein precipitation and analyzed by liquid chromatography equipped with a C18 column interfaced with a triple quadrupole tandem mass spectrometer using ESI as the ionization source in the negative ion mode. The mobile phase consisted of water (0.01 % formic acid)-methanol (57 : 43, V/V) at the flow rate of 0.3 mL·min(-1). The analytes and internal standard acteoside were both detected by use of multiple reaction monitoring mode. The total run time was 6.0 min. The method was linear in the concentration range of 2.5-500.0 ng·mL(-1) and the lower limit of quantifiation (LLOQ) was 2.5 ng·mL(-1). The intra-day and inter-day relative standard deviations across three validation runs over the entire concentration range were less than 9.8 %. The accuracy determined at three concentrations was within ± 6.1% in terms of relative error. In conclusion, this assay offers advantages in terms of expediency and suitability for the analysis of ligupurpuroside B and ligupurpuroside C in various biological fluids.
3.Antioxidative activities of phenylethanoid glycosides from Ligustrum purpurascens.
Wong IY;He ZD;Huang Y;Chen ZY J Agric Food Chem. 2001 Jun;49(6):3113-9.
Tea and kudingcha (bitter tea) are the two most popular beverages consumed in China. Tea derived from the leaves of Camellia sinensis has been well studied for its various health benefits, but there are very limited data on the biological activities of bitter tea derived from the leaves of Ligustrum purpurascens (LP). The present study was carried out to characterize the antioxidants present in the bitter tea brewed from the leaves of LP. It was found that the crude glycoside fraction possessed strong protection against oxidation of human low-density lipoprotein (LDL). The column chromatographic separation led to the isolatation of five phenylethanoid glycosides, namely, acteoside, ligupurpuroside A, cis-ligupurpuroside B, trans-ligupurpuroside B, and osmanthuside B. When acteoside was heated in the boiling water, it was isomerized to form isoacteoside. Acteoside, isoacteoside, and ligupurpuroside A purified from LP were protective, whereas cis-ligupurpuroside B, trans-ligupurpuroside B, and osmanthuside B exhibited no protection to human LDL from Cu(2+)-medicated oxidation. Acteoside, isoacteoside, and ligupurpuroside A were also effective in preventing the peroxyl free radical-induced oxidation of alpha-tocopherol in human LDL.
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CAS 147396-02-9 Ligupurpuroside B

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