1. Functional synergy of α-helical antimicrobial peptides and traditional antibiotics against Gram-negative and Gram-positive bacteria in vitro and in vivo
Q. Feng & Y. Huang & M. Chen & G. Li & Y. Chen. Eur J Clin Microbiol Infect Dis (2015) 34:197–204
Complicated skin infections, such as post-surgical and traumatic wound infections, severe carbunculosis, as well as erysipelas, are often caused by Gram-positive bacteria such as S. aureus and S. pyogenes. Levofloxacin hydrochloride was widely used in clinical applications against infections. Peptide PL-5 exhibited the best overall synergistic activity with levofloxacin hydrochloride against Gram-positive bacteria in vitro, that is, combining AMP PL-5 with levofloxacin hydrochloride produced potent synergistic effects (the FIC index was 0.078, Table S1). Thus, this drug combination was used to evaluate the antimicrobial effects, in vivo, of a mouse wound model infected with S. aureus. Based on the preliminary experiments (data not shown), mice were treated with two different doses of PL-5 and levofloxacin hydrochloride to investigate the synergistic efficacy (Table 3). The bacteriostatic rate was used to evaluate the antimicrobial efficacy.