KU-55933 - CAS 587871-26-9
Catalog number: 587871-26-9
Category: Inhibitor
Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Molecular Formula:
C21H17NO3S2
Molecular Weight:
395.49
COA:
Inquire
Targets:
ATM
Description:
KU-55933 is an ATM inhibitor,  which blocks the phosphorylation of Akt induced by insulin and insulin-like growth factor I in cancer cells that exhibit abnormal Akt activity. Moreover, KU-55933 inhibits cancer cell proliferation by inducing G(1) cell cycle arrest. It does so through the downregulation of the synthesis of cyclin D1, a protein known to be elevated in a variety of tumors. In addition, KU-55933 treatment during serum starvation triggers apoptosis in these cancer cells. Research results suggest that KU-55933 may be a novel chemotherapeutic agent targeting cancer resistant to traditional chemotherapy or immunotherapy due to aberrant activation of Akt. Furthermore, KU-55933 completely abrogates rapamycin-induced feedback activation of Akt. Combination of KU-55933 and rapamycin not only induces apoptosis, which is not seen in cancer cells treated only with rapamycin, but also shows better efficacy in inhibiting cancer cell proliferation than each drug alone.
Appearance:
White to off-white solid powder
Synonyms:
KU 55933; KU-55933; KU55933.
MSDS:
Inquire
InChIKey:
XRKYMMUGXMWDAO-UHFFFAOYSA-N
InChI:
InChI=1S/C21H17NO3S2/c23-14-12-16(25-20(13-14)22-8-10-24-11-9-22)15-4-3-7-19-21(15)27-18-6-2-1-5-17(18)26-19/h1-7,12-13H,8-11H2
Canonical SMILES:
C1COCCN1C2=CC(=O)C=C(O2)C3=C4C(=CC=C3)SC5=CC=CC=C5S4
Current Developer:
Merck
1.The influence of ATM, ATR, DNA-PK inhibitors on the cytotoxic and genotoxic effects of dibenzo[def,p]chrysene on human hepatocellular cancer cell line HepG2.
Spryszyńska S1, Smok-Pieniążek A2, Ferlińska M3, Roszak J4, Nocuń M5, Stępnik M6. Mutat Res Genet Toxicol Environ Mutagen. 2015 Sep;791:12-24. doi: 10.1016/j.mrgentox.2015.07.008. Epub 2015 Jul 26.
The effect of inhibitors of phosphatidylinositol-3-kinase related kinases (PIKK): ataxia-telangiectasia mutated (ATM), ATM- and Rad3-related (ATR) and DNA-dependent protein kinase (DNA-PK) on the response of HepG2 human liver cancer cells to dibenzo[def,p]chrysene (DBC) was investigated. High cytotoxicity of DBC (IC50=0.1μM) was observed after 72h incubation. PIKK inhibitors: KU55933 (5μM), NU7026 (10μM) or caffeine (1 and 2mM) when used alone did not significantly influence the cytotoxicity. However, two combinations: KU55933/NU7026 and caffeine/NU7026 significantly increased HepG2 viability (by 25%) after treatment with DBC at 0.5μM. The cytoprotective effect was confirmed by cell cycle and apoptosis/necrosis analysis. DNA damage level after exposure to DBC assessed by comet assay (single strand breaks) showed a long persistence and significant decrease after incubation of the cells in the presence the inhibitors (the combination of KU55933+NU7026 showed the strongest effect).
2.Changes in the response of MCF-7 cells to ionizing radiation after the combination of ATM and DNA-PK inhibition.
Ćmielová J1, Havelek R, Vávrová J, Řezáčová M. Med Oncol. 2015 May;32(5):138. doi: 10.1007/s12032-015-0591-1. Epub 2015 Mar 24.
The aim of the present study is to evaluate the role of ATM (KU55933) and DNA-PK (NU7441) inhibitors in the repair of double-strand breaks and downstream signaling of DNA damage introduced by ionizing radiation. The irradiation of MCF-7 cells alone increased the proportion of cells in the G1 phase in comparison with mock-treated cells. After ATM inhibitor pretreatment, the cells were more accumulated in the G2 phase, whereas DNA-PK inhibitor application increased the percentage of cells in the G1 phase. ATM and DNA-PK inhibitor application alone increased the sensitivity of MCF-7 cells to ionizing radiation; however, combining both inhibitors together resulted in a further enhancement of cell death. Unexpectedly, combining both inhibitors decreased the percentage of senescent cells and increased G2 cell cycle arrest 3 days after treatment. After irradiation, the p21 protein was increased and Chk1 and Chk2 were activated. These proteins were not increased in cells pretreated with the ATM inhibitor prior to ionizing radiation exposure, albeit DNA-PK inhibitor application did not affect the amount of proteins detected.
3.Imatinib inhibits inactivation of the ATM/ATR signaling pathway and recovery from adriamycin/doxorubicin-induced DNA damage checkpoint arrest.
Morii M1, Fukumoto Y1, Kubota S1, Yamaguchi N1, Nakayama Y1,2, Yamaguchi N1. Cell Biol Int. 2015 Aug;39(8):923-32. doi: 10.1002/cbin.10460. Epub 2015 Apr 27.
The DNA damage checkpoint arrests cell cycle progression to allow time for DNA repair. After completion of DNA repair, checkpoint activation is terminated, and cell cycle progression is resumed in a process called checkpoint recovery. The activation of the checkpoint has been studied in depth, but little is known about recovery from the DNA damage checkpoint. Recently we showed that Src family kinases promote recovery from the G2 DNA damage checkpoint. Here we show that imatinib inhibits inactivation of ATM/ATR signaling pathway to suppress recovery from Adriamycin/doxorubicin-induced DNA damage checkpoint arrest. Imatinib and pazopanib, two distinct inhibitors of PDGFR/c-Kit family kinases, delayed recovery from checkpoint arrest and inhibited the subsequent S-G2-M transition after Adriamycin exposure. By contrast, imatinib and pazopanib did not delay the recovery from checkpoint arrest in the presence of an ATM/ATR inhibitor caffeine. Consistently, imatinib induced a persistent activation of ATR-Chk1 signaling.
4.Induction of ROS-independent DNA damage by curcumin leads to G2/M cell cycle arrest and apoptosis in human papillary thyroid carcinoma BCPAP cells.
Zhang L1, Cheng X2, Gao Y2, Bao J2, Guan H3, Lu R4, Yu H2, Xu Q5, Sun Y5. Food Funct. 2016 Jan 20;7(1):315-25. doi: 10.1039/c5fo00681c.
Previously we found that curcumin, the active constituent of dietary spice turmeric, showed potent inhibitory effects on the cell growth of thyroid cancer cells. However, the detailed anti-cancer mechanism of curcumin is still unknown. In this study, we have reported that curcumin induces significant DNA damage in human papillary thyroid carcinoma BCPAP cells in a dose-dependent manner as evidenced by the upregulated phosphorylation of H2A.X at Ser139, which was further confirmed by the long tails in the comet assay and the increase in the number of TUNEL-positive cells. Subsequently, curcumin treatment caused a significant accumulation of cells at the G2/M phase that eventually resulted in a caspase-dependent apoptosis in BCPAP cells. DNA agarose gel electrophoresis revealed that curcumin-induced DNA damage in BCPAP cells was independent of DNA conformational change. Pretreatment with reactive oxygen species (ROS) scavengers failed to block the phosphorylation of H2A.
Molecular Weight Calculator Molarity Calculator Solution Dilution Calculator

Related ATM Products


CAS 587871-26-9 KU-55933

KU-55933
(CAS: 587871-26-9)

KU-55933 is an ATM inhibitor,  which blocks the phosphorylation of Akt induced by insulin and insulin-like growth factor I in cancer cells that exhibit abnormal...

CAS 905973-89-9 CGK733

CGK733
(CAS: 905973-89-9)

CGK733, a thiourea-containing compound, was originally identified as a specific inhibitor of ATR and ATM kinases (IC50 = ~ 200 nM). In prematurely senescent bre...

CAS 925701-46-8 KU-60019

KU-60019
(CAS: 925701-46-8)

KU-60019 is a potent and reversible inhibitor of ATM kinase with IC50 of 6.3 nM. It is an improved analogue of KU-55933. It exhibits little to no nonspecific ta...

CAS 19545-26-7 Wortmannin

Wortmannin
(CAS: 19545-26-7)

Wortmannin, a steroid metabolite of the fungi Penicillium funiculosum, Talaromyces (Penicillium) wortmannii, is a specific, covalent inhibitor of phosphoinositi...

CAS 1080622-86-1 CP-466722

CP-466722
(CAS: 1080622-86-1)

CP-466722 is a potent and reversible ATM inhibitor, does not affect ATR and inhibits PI3K or PIKK family members in cells.

BAY-1895344 hydrochloride

BAY-1895344 is a potent, orally available and highly selective ATR inhibitor (IC50= 7 nM), which potently inhibits proliferation of a broad spectrum of human tu...

Chemical Structure

CAS 587871-26-9 KU-55933

Quick Inquiry

Verification code

Featured Items