Indo-1 AM - CAS 112926-02-0
Molecular Formula:
C47H51N3O22
Molecular Weight:
1009.9
COA:
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Application\Fluorophore:
Ion Indicators and Sensors
Description:
Indo-1 AM is a cell-permeant acetoxymethyl ester of indo-1, a ratiometric fluorescent calcium indicator. Indo-1 AM is hydrolyzed by intracellular esterases to release Indo-1 in cells. Indo-1 is commonly used in flow cytometry. The emission maximum shifts from 475-485 nm without calcium to 400-410 nm when indo-1 binds calcium.
Purity:
≥90%
Appearance:
Solid Powder
Synonyms:
Indo-1 Acetoxymethyl ester; Indo-1 pentaacetoxymethyl ester; 2-[4-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-Indole-6-carboxylic acid, (acetyloxy)methyl ester
Storage:
Store at -20°C
MSDS:
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Excitation:
349-364 nm
Emission:
475-485 nm (no Ca2+), 400-410 nm (Ca2+-bound)
InChIKey:
CAWBRCOBJNWRLK-UHFFFAOYSA-N
InChI:
InChI=1S/C47H51N3O22/c1-28-7-11-39(49(19-43(56)68-23-63-29(2)51)20-44(57)69-24-64-30(3)52)41(15-28)61-13-14-62-42-18-35(37-16-34-8-9-36(17-38(34)48-37)47(60)72-27-67-33(6)55)10-12-40(42)50(21-45(58)70-25-65-31(4)53)22-46(59)71-26-66-32(5)54/h7-12,15-18,48H,13-14,19-27H2,1-6H3
Canonical SMILES:
CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=CC4=C(N3)C=C(C=C4)C(=O)OCOC(=O)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
1.Caribbean maitotoxin elevates [Ca(2+)]i and activates non-selective cation channels in HIT-T15 cells.
Lu XZ1, Deckey R, Jiao GL, Ren HF, Li M. World J Diabetes. 2013 Jun 15;4(3):70-5. doi: 10.4239/wjd.v4.i3.70.
AIM: To investigate the cytotoxic mechanism of caribbean maitotoxin (MTX-C) in mammalian cells.
2.Choice of anesthetic combination determines Ca2+ leak after ischemia-reperfusion injury in the working rat heart: favorable versus adverse combinations.
Zaugg M1, Wang L, Zhang L, Lou PH, Lucchinetti E, Clanachan AS. Anesthesiology. 2012 Mar;116(3):648-57. doi: 10.1097/ALN.0b013e318247225a.
BACKGROUND: There is a lack of studies investigating cardioprotection by common combinations of anesthetics. However, because a general anesthetic consists of a mixture of drugs with potentially interfering effects on signaling and cytoprotection, the most favorable combination should be used.
3.Fluorescent assessment of intracellular calcium in individual cells.
Cox DP1, White CC, Kavanagh TJ. Methods Mol Biol. 2011;758:239-51. doi: 10.1007/978-1-61779-170-3_16.
Calcium is an important intracellular ion involved in numerous cell processes. There are multiple factors that contribute to the release of Ca²⁺. Some factors induce release as part of intracellular signaling cascades, while others result in unwanted changes to both basal and inducible Ca²⁺ levels. The accurate measurement of intracellular Ca²⁺ is, therefore, an important tool in neurotoxicology for assessing compounds/substances that disrupt Ca²⁺ homeostasis. Fluorescent, Ca²⁺-sensitive probes Indo-1 and Fluo-3 allow for the quantification of intracellular Ca²⁺ in individual cells to determine what effects neurotoxins have on both basal and stimulus-dependent Ca²⁺ concentrations.
4.New experimental evidence for mechanism of arrhythmogenic membrane potential alternans based on balance of electrogenic I(NCX)/I(Ca) currents.
Wan X1, Cutler M, Song Z, Karma A, Matsuda T, Baba A, Rosenbaum DS. Heart Rhythm. 2012 Oct;9(10):1698-705. doi: 10.1016/j.hrthm.2012.06.031. Epub 2012 Jun 19.
BACKGROUND: Computer simulations have predicted that the balance of various electrogenic sarcolemmal ion currents may control the amplitude and phase of beat-to-beat alternans of membrane potential (V(m)). However, experimental evidence for the mechanism by which alternans of calcium transients produces alternation of V(m) (V(m)-ALT) is lacking.
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CAS 112926-02-0 Indo-1 AM

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