1.Adaption of the [3H]-hypoxanthine uptake assay for in vitro cultured Plasmodium knowlesi malaria parasites.
Arnold MS1, Engel JA1, Chua MJ1, Fisher GM1, Skinner-Adams TS1, Andrews KT2. Antimicrob Agents Chemother. 2016 Apr 25. pii: AAC.02948-15. [Epub ahead of print]
The zoonotic malaria parasite Plasmodium knowlesi has recently been established in continuous in vitro culture. Here the P. falciparum [3H]-hypoxanthine-uptake assay was adapted for P. knowlesi and used to determine the sensitivity of this parasite to chloroquine, cycloguanil and clindamycin. Data demonstrate that P. knowlesi is sensitive to all drugs with IC50s consistent with those obtained with P. falciparum This assay provides a platform to use P. knowlesi in vitro for drug discovery.