1.A one-step synthesis of water-soluble MoS2 quantum dots via hydrothermal method as fluorescent probe for hyaluronidase detection.
Gu W, Yan Y, Zhang C, Ding C, Xian Y. ACS Appl Mater Interfaces. 2016 Apr 15. [Epub ahead of print]
In this work, a bottom-up strategy is developed to synthesize water-soluble molybdenum disulfide quantum dots (MoS2 QDs) through a simple, one-step hydrothermal method using ammonium tetrathiomolybdate ((NH4)2MoS4) as precursor and hydrazine hydrate as the reducing agent. The as-synthesized MoS2 QDs are few-layered with a narrow size-distribution, and the average diameter is about 2.8 nm. The resultant QDs show excitation-dependent blue fluorescence due to the polydispersity of QDs. Moreover, the fluorescence can be quenched by hyaluronic acid (HA) functionalized gold nanoparticles (AuNPs) through a photoinduced electron transfer mechanism. Hyaluronidase (HAase), an endoglucosidases, can cleave HA into proangiogenic fragments and lead to the aggregation of gold nanoparticles. As a result, the electron transfer is blocked and fluorescence is recovered. Based on this principle, a novel fluorescence sensor for HAase is developed with a linear ranging from 1 to 50 U/mL and a detection limit of 0.
2.Self assembled hyaluronic acid nanoparticles as a potential carrier for targeting the inflamed intestinal mucosa.
Vafaei SY1, Esmaeili M2, Amini M3, Atyabi F4, Ostad SN5, Dinarvand R6. Carbohydr Polym. 2016 Jun 25;144:371-81. doi: 10.1016/j.carbpol.2016.01.026. Epub 2016 Jan 25.
To develop a nanoparticulate drug carrier for targeting of the inflamed intestinal mucosa, amphiphilic hyaluronic acid (HA) conjugates were synthesized, which could form self-assembled nanoparticles (NPs) in aqueous solution and budesonide (BDS) was loaded into the HANPs. Their particle sizes were in the range of 177 to 293nm with negative surface charge. The model of inflammatory CACO-2 cells was utilized to investigate the therapeutic potential of budesonide loaded HA nanocarriers. The highest expression of CD44 receptors was found on inflamed Caco-2 cells, as determined by flow cytometry. FITC-labeled HANPs revealed greater uptake in inflamed CACO-2 cells compared to untreated CACO-2 and CD44-negative cell lines, NIH3T3. BDS loaded HANPs displayed almost no toxicity indicating HANPs are excellent biocompatible nano-carriers. BDS loaded HANPs demonstrated higher anti-inflammatory effect on IL-8 and TNF-α secretion in inflamed cell model compared to the same dose of free drug.
3.Low-molecular-weight polymer-drug conjugates for synergistic anticancer activity of camptothecin and doxorubicin combinations.
Camacho KM1, Menegatti S2, Mitragotri S1. Nanomedicine (Lond). 2016 Apr 15. [Epub ahead of print]
BACKGROUND: High-molecular-weight (MW) polymers (>50,000 Da) can be conjugated to chemotherapy drugs in order to improve their tumor accumulation, while low MW polymers ≤10,000 Da are often overlooked due to faster plasma clearance. Small polymers, however, may facilitate deeper tumor penetration.
4.Prolyl-4-Hydroxylases Inhibitor Stabilizes HIF-1α and Increases Mitophagy to Reduce Cell Death After Experimental Retinal Detachment.
Liu H1, Zhu H2, Li T2, Zhang P2, Wang N2, Sun X3. Invest Ophthalmol Vis Sci. 2016 Apr 1;57(4):1807-1815. doi: 10.1167/iovs.15-18066.
Purpose: This study investigated the neuroprotective effect against photoreceptor cell death using prolyl-4-hydroxylases inhibitor (PHI), an HIF-1α stabilizer, in experimental retinal detachment (RD).