GW-1100 - CAS 306974-70-9
Catalog number: 306974-70-9
Category: Inhibitor
Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Molecular Formula:
C27H25FN4O4S
Molecular Weight:
520.58
COA:
Inquire
Targets:
GPR40
Description:
A cell-permeable pyrimidinylmethyl-pyrimidinone compound that acts as potent, reversible and non-competitive GPR40 antagonist. Shown to significantly reverse glucose-induced insulin secretion potentiated by GW9508 and only partially reduce linoleic acid effect in MIN6 cells.
Purity:
>98%
Synonyms:
GW 1100; GW-1100; GW1100.
MSDS:
Inquire
InChIKey:
PTPNCCWOTBBVJR-UHFFFAOYSA-N
InChI:
InChI=1S/C27H25FN4O4S/c1-3-35-25(34)20-7-11-23(12-8-20)32-16-21(13-19-14-29-26(30-15-19)36-4-2)24(33)31-27(32)37-17-18-5-9-22(28)10-6-18/h5-12,14-16H,3-4,13,17H2,1-2H3
Canonical SMILES:
CCOC1=NC=C(C=N1)CC2=CN(C(=NC2=O)SCC3=CC=C(C=C3)F)C4=CC=C(C=C4)C(=O)OCC
1.Tumor necrosis factor α decreases glucagon-like peptide-2 expression by up-regulating G-protein-coupled receptor 120 in Crohn disease.
Tsukahara T;Watanabe K;Watanabe T;Yamagami H;Sogawa M;Tanigawa T;Shiba M;Tominaga K;Fujiwara Y;Maeda K;Hirakawa K;Arakawa T Am J Pathol. 2015 Jan;185(1):185-96. doi: 10.1016/j.ajpath.2014.09.010. Epub 2014 Nov 5.
Glucagon-like peptide (GLP)-2, secreted by L cells in the small intestine, has anti-inflammatory effects in the gastrointestinal tract. A GLP-2 analogue has been an effective treatment for Crohn disease (CD). G-protein-coupled receptor (GPR) 40 and GPR120 are probably involved in GLP-2 production, the mechanisms of which remain unclear. In our experiments, normal ileal mucosa expressed GPR40, but rarely expressed GPR120. However, both GPRs were overexpressed in the L cells of the inflamed ileal mucosa of CD patients. Mucosal inflammation induced the overexpression of GPR40, GPR120, and several inflammatory cytokines, with correlations between ileal concentrations of tumor necrosis factor (TNF)-α and GPR expression levels; however, inflammation did not induce the expression of proglucagon, a precursor of GLP-2 in CD patients. In rat L cells and GLUTag cells, TNF-α treatment increased GPR120 mRNA expression without affecting GPR40 mRNA expression. Dual agonists of GPR40 and GPR120, GW9508 and linoleic acid, respectively, increased GLP-2 production from L cells, but these agonists decreased it in the presence of TNF-α. The GPR40 antagonist, GW1100, inhibited the GW9508-induced increase in GLP-2 production, and silencing GPR120 resulted in further elevation of GLP-2 production.
2.Cloning, identification and functional characterization of bovine free fatty acid receptor-1 (FFAR1/GPR40) in neutrophils.
Manosalva C;Mena J;Velasquez Z;Colenso CK;Brauchi S;Burgos RA;Hidalgo MA PLoS One. 2015 Mar 19;10(3):e0119715. doi: 10.1371/journal.pone.0119715. eCollection 2015.
Long chain fatty acids (LCFAs), which are ligands for the G-protein coupled receptor FFAR1 (GPR40), are increased in cow plasma after parturition, a period in which they are highly susceptible to infectious diseases. This study identified and analyzed the functional role of the FFAR1 receptor in bovine neutrophils, the first line of host defense against infectious agents. We cloned the putative FFAR1 receptor from bovine neutrophils and analyzed the sequence to construct a homology model. Our results revealed that the sequence of bovine FFAR1 shares 84% identity with human FFAR1 and 31% with human FFAR3/GPR41. Therefore, we constructed a homology model of bovine FFAR1 using human as the template. Expression of the bovine FFAR1 receptor in Chinese hamster ovary (CHO)-K1 cells increased the levels of intracellular calcium induced by the LCFAs, oleic acid (OA) and linoleic acid (LA); no increase in calcium mobilization was observed in the presence of the short chain fatty acid propionic acid. Additionally, the synthetic agonist GW9508 increased intracellular calcium in CHO-K1/bFFAR1 cells. OA and LA increased intracellular calcium in bovine neutrophils. Furthermore, GW1100 (antagonist of FFAR1) and U73122 (phospholipase C (PLC) inhibitor) reduced FFAR1 ligand-induced intracellular calcium in CHO-K1/bFFAR1 cells and neutrophils.
3.Differential free fatty acid receptor-1 (FFAR1/GPR40) signalling is associated with gene expression or gelatinase granule release in bovine neutrophils.
Mena SJ;Manosalva C;Carretta MD;Teuber S;Olmo I;Burgos RA;Hidalgo MA Innate Immun. 2016 Aug;22(6):479-89. doi: 10.1177/1753425916656765. Epub 2016 Jun 30.
Fatty acids have been recognized as regulators of immune function in addition to their known metabolic role. Long-chain fatty acids bind free fatty acid receptor (FFAR)-1/GPR40, which is expressed on bovine neutrophils, and increase responses such as granule release and gene expression. In this study, we investigated the molecular mechanisms governing the up-regulation of cyclooxygenase-2 (COX-2) and IL-8, as well as matrix metalloproteinase (MMP)-9 granule release in FFAR1/GPR40 agonist-stimulated neutrophils. Our results showed that natural (oleic and linoleic acid) and synthetic (GW9508) FFAR1/GPR40 agonists increased ERK1/2, p38 MAPK and Akt phosphorylation, and that the FFAR1/GPR40 antagonist GW1100 reduced these responses. We evaluated the levels of IκBα, a component of the classical activation pathway of the transcription factor NF-κB, and we observed IκBα reduction after stimulation with FFAR1/GPR40 agonists, an effect that was inhibited by GW1100 or the inhibitors UO126, SB203580 or LY294002. FFAR1/GPR40 agonists increased COX-2 and IL-8 expression, which was inhibited by GW1100 and an NF-κB inhibitor. Finally, the FFAR1/GPR40 agonist-induced MMP-9 granule release was reduced by GW1100 and UO126.
Molecular Weight Calculator Molarity Calculator Solution Dilution Calculator

Related GPR40 Products


CAS 1236109-67-3 TUG-469

TUG-469
(CAS: 1236109-67-3)

This active molecular is a free fatty acid (FFA) 1 receptor agonist. TUG-469 is more than two hundred fold selective for FFA1 over FFA4. TUG-469 can potential u...

CAS 1011531-27-3 AM4668

AM4668
(CAS: 1011531-27-3)

AM4668 is a potent and selective G-protein coupled receptor 40(GPR40) agonist with EC50 value of 3.6 nM in an IP3 assay in GPR40 transfected A9 cells. It is mor...

CAS 865231-46-5 AMG 837

AMG 837
(CAS: 865231-46-5)

AMG 837 sodium salt is a potent GPR40 agonist with a superior pharmacokinetic profile and robust glucose-dependent stimulation of insulin secretion in rodents. ...

CAS 87272-20-6 MEDICA 16

MEDICA 16
(CAS: 87272-20-6)

MEDICA 16 is a selective free fatty acid 1 receptor agonist. It is also an ATP citrate lyase inhibitor. MEDICA 16 shows hypolipidemic and antidiabetogenic effec...

CAS 1259389-38-2 AMG 837 calcium hydrate

AMG 837 calcium hydrate
(CAS: 1259389-38-2)

AMG 837 calcium hydrate is a potent GPR40 agonist(EC50=13 nM) with a superior pharmacokinetic profile and robust glucose-dependent stimulation of insulin secret...

CAS 1000413-72-8 TAK-875

TAK-875
(CAS: 1000413-72-8)

TAK-875 is a potent, selective, and orally bioavailable GPR40 agonist, with a pharmacokinetic profile enabling long-acting drug efficacy.

CAS 1309435-60-6 GPR40 Activator 1

GPR40 Activator 1
(CAS: 1309435-60-6)

GPR40 Activator 1 is a potent GPR40 activator for treatment of type 2 diabetes.

CAS 1402601-82-4 TUG-770

TUG-770
(CAS: 1402601-82-4)

TUG-770 displayed excellent physicochemical and in vitro ADME properties, with good aqueous solubility, good chemical stability, low lipophilicity, and decrease...

Chemical Structure

CAS 306974-70-9 GW-1100

Quick Inquiry

Verification code

Featured Items