Protein, antibodies, peptides, nucleic acids, ligands, synthetic oligonucleotides and other biomolecules, labeling with fluorescent molecules that allows the sensing and visualizing of protein dynamics, localization, and protein-protein interactions, is an invaluable technique to understand protein functions and networks in living cells.
Various labeling techniques are developed in BOC Sciences such as enzymatic labeling, protein labeling, genetic labeling and quantum dot are widely utilized to meet our customers’ need.
Please find our labeling methods for tracking biomolecules as below:
1. Enzymatic labeling
Incorporate functionalities into proteins at specific sites via enzymatic reactions is one of the most important approaches for protein labeling. The incorporated tags then enable reactions that are chemoselective, whose functional groups not only are inert in biological media, but also do not occur natively in proteins or other macromolecules.
2. Chemical labeling
Chemical labeling or the use of chemical tags could offer a wider range of colors and photo chemical properties. Also, chemical tags are preferred over fluorescent proteins due to the architectural and size limitations of the fluorescent protein's characteristic b-barrel.
3. Protein labeling
A short tag is used to minimize disruption of protein folding and function in protein labeling. Sometimes, transition metals are also used to link specific residues in the tags to site-specific targets such as the N, C-termini, or internal sites within the protein.
4. Genetic labeling
Genetic labeling, also known as chromosome painting, is a genetic labeling technique utilizes probes that are specific for chromosomal sites along the length of a chromosome. This technique allows abnormalities such as deletions and duplications to be revealed.
5. Quantum dot labeling
The tunable absorption spectrum and high extinction coefficients of quantum dots have made them attractive for light harvesting technologies such as labeling.
With the proliferation of fluorescent proteins in recent years, there are many fluorescent protein combinations that can be imaged together. Genetic encoding of fluorescent proteins (FPs) such as Ethidium bromide, fluorescein and green fluorescent protein are the most popular technique for protein labeling because of some important advantages, including high labeling specificity and simplicity. BOC Sciences offers a full spectrum of labeling kits to prepare conjugates with enzymes, fluorophore, biotin as well as use with immunochemistry, fluorescence in situ hybridization (FISH), cell tracing and receptor labeling.
Here is the products and services that we provide:
|Name||Quantity||Ex(nm)||Em(nm)||Shelf life||Lead time|
|RTGreen nucleic acid gel stain *20× concentrate in water*||500 μL||495||520||12 months||1 week|
|RTGreen nucleic acid gel stain *20× concentrate in water*||1 mL||495||520||12 months||1 week|
|2× SYBR Green qPCR Mix||50x 50μL||497||525||6 months||1 week|
|2× SYBR Green qPCR Mix||200x 50μL||497||525||6 months||1 week|
|ROX Reference Dye||50 μL||568||591||12 months||1 week|
|ROX Reference Dye||200 μL||568||591||12 months||1 week|
|PicaGreen dsDNA Assay Kit *2000 assays*||10×100 μL||488||520||12months||1 week|
|PicaGreen dsDNA Assay Kit *2000 assays*||1 mL||488||520||12months||1 week|
|OligoGreen ssDNA Assay Kit *2000 assays*||10×100 μL||488||520||12months||1 week|
|OligoGreen ssDNA Assay Kit *2000 assays*||1 mL||488||520||12months||1 week|
|RibaGreen RNA Assay Kit *2000 assays*||10×100 μL||488||520||12months||1 week|
|RibaGreen RNA Assay Kit *2000 assays*||1 mL||488||520||12months||1 week|
With advanced technology and rich experience, we will offer you the best service in this area. Please do not hesitate to contact us and let us know your need.