Fluo-3 AM - CAS 121714-22-5
Molecular Formula:
C51H50Cl2N2O23
Molecular Weight:
1129.9
COA:
Inquire
Application\Fluorophore:
Ion Indicators and Sensors
Description:
Fluo-3 is a visable wavelength calcium indicator commonly used in flow cytometry (FC) and cell-based experiments to detect changes in intracellular calcium levels. Fluo-3 AM is cell-permeable and can be cleaved to release fluo-3 in cells.
Purity:
≥90%
Synonyms:
Fluo-3 Acetoxymethyl ester; N-[4-[6-[(acetyloxy)methoxy]-2,7-dichloro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-glycine, (acetyloxy)methyl ester
Storage:
Store at -20°C
MSDS:
Inquire
Excitation:
488 nm
Emission:
526 nm
InChIKey:
PTPUOMXKXCCSEN-UHFFFAOYSA-N
InChI:
InChI=1S/C51H50Cl2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2)56/h7-10,13-18H,11-12,19-27H2,1-6H3
Canonical SMILES:
CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)Cl)OCOC(=O)C)Cl)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
1.Resveratrol protects PC12 cells against OGD/R-induced apoptosis via the mitochondrial-mediated signaling pathway.
Liu X1, Zhu X2, Chen M1, Ge Q1, Shen Y1, Pan S3. Acta Biochim Biophys Sin (Shanghai). 2016 Mar 8. pii: gmw011. [Epub ahead of print]
In this study, we investigated the neuroprotective potential of resveratrol against oxygen glucose deprivation/reoxygenation (OGD/R)-induced apoptotic damages in well-differentiated PC12 cells and the underlying mechanisms. Cells were incubated under normal condition or OGD/R in the presence or absence of 10 μM resveratrol. Cell viability was determined with methyl-thiazolyl-tetrazolium (MTT) assay. Apoptotic ratio was determined with Hoechst 33342 staining and Annexin V-FITC/PI double staining. Oxidative stress was evaluated by measuring the intracellular reactive oxygen species (ROS), the mitochondrial superoxide, the malondialdehyde (MDA) content, and the activities of superoxide dismutase (SOD) and catalase (CAT). The intracellular calcium ([Ca2+]i) was estimated by Fluo-3/AM. The mitochondrial membrane potential (MMP) was evaluated by 5,5',6,6'-tetrachloro-1,1,3,3'-tetraethyl-benzimidazolyl-carbocyanine iodide (JC-1) and rhodamine 123 (Rh123).
2.Bile Acid-Induced Suicidal Erythrocyte Death.
Lang E, Pozdeev VI, Gatidis S, Qadri SM, Häussinger D, Kubitz R, Herebian D, Mayatepek E, Lang F, Lang KS, Lang PA. Cell Physiol Biochem. 2016 Apr 7;38(4):1500-1509. [Epub ahead of print]
BACKGROUND/AIMS: In nucleated cells, bile acids may activate cation channels subsequently leading to entry of Ca2+. In erythrocytes, increase of cytosolic Ca2+ activity triggers eryptosis, the suicidal death of erythrocytes characterized by phosphatidylserine exposure at the cell surface and cell shrinkage. Eryptosis is triggered by bile duct ligation, an effect partially attributed to conjugated bilirubin. The present study explored, whether bile acids may stimulate eryptosis.
3.[Effect of Artesunate on Proliferation, Cell Cycle and Apoptosis of SKM-1 Cells and Its Underlying Mechanisms].
Qiao SK1, Wang Y2, Niu ZY1, Tan JM1, Wang JL1. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2016 Feb;24(1):131-7. doi: 10.7534/j.issn.1009-2137.2016.01.026.
OBJECTIVE: To investigate the effects of artesunate (ART) on proliferation, cell cycle and apoptosis of SKM-1 cells in vitro and to explore the underlying mechanisms.
4.Dietary nitrate improves cardiac contractility via enhanced cellular Ca(2+) signaling.
Pironti G1, Ivarsson N1, Yang J2, Farinotti AB1, Jonsson W1, Zhang SJ1, Bas D1, Svensson CI1, Westerblad H1, Weitzberg E1, Lundberg JO1, Pernow J2,3, Lanner J1, Andersson DC4,5,6. Basic Res Cardiol. 2016 May;111(3):34. doi: 10.1007/s00395-016-0551-8. Epub 2016 Apr 12.
The inorganic anion nitrate (NO3 (-)), which is naturally enriched in certain vegetables (e.g., spinach and beetroot), has emerged as a dietary component that can regulate diverse bodily functions, including blood pressure, mitochondrial efficiency, and skeletal muscle force. It is not known if dietary nitrate improves cardiac contractility. To test this, mice were supplemented for 1-2 weeks with sodium nitrate in the drinking water at a dose similar to a green diet. The hearts from nitrate-treated mice showed increased left ventricular pressure and peak rate of pressure development as measured with the Langendorff heart technique. Cardiomyocytes from hearts of nitrate-treated and control animals were incubated with the fluorescent indicator Fluo-3 to measure cytoplasmic free [Ca(2+)] and fractional shortening. Cardiomyocytes from nitrate-treated mice displayed increased fractional shortening, which was linked to larger Ca(2+) transients.
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CAS 121714-22-5 Fluo-3 AM

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