Coelenterazine - CAS 55779-48-1
Catalog number: 55779-48-1
Category: Inhibitor
Not Intended for Therapeutic Use. For research use only.
Molecular Formula:
C26H21N3O3
Molecular Weight:
423.46
COA:
Inquire
Targets:
reporter genes in BRET, ELISA and HTS techniques
Description:
Coelenterazine is a luminescent enzyme substrate, used for monitoring reporter genes in BRET, ELISA and HTS techniques.
Publictions citing BOC Sciences Products
  • >> More
Purity:
98%
Appearance:
Solid powder
Synonyms:
Oplophorus luciferin; Coelenterazine native; CLZN; Coelenterazine, native;
Solubility:
DMSO,84mg/mL(198.36mM);Ethanol,1mg/mL(2.36mM);Water,<1mg/mL(<1mM)
Storage:
3 years -20℃powder;6 months-80℃in solvent
MSDS:
Inquire
Shelf Life:
2 years if stored properly
Canonical SMILES:
C1=CC=C(C=C1)CC2=C3N=C(C(=O)N3C=C(N2)C4=CC=C(C=C4)O)CC5=CC=C(C=C5)O
1.Expression, purification and luminescence properties of coelenterazine-utilizing luciferases from Renilla, Oplophorus and Gaussia: comparison of substrate specificity for C2-modified coelenterazines.
Inouye S1, Sahara-Miura Y, Sato J, Iimori R, Yoshida S, Hosoya T. Protein Expr Purif. 2013 Mar;88(1):150-6. doi: 10.1016/j.pep.2012.12.006. Epub 2012 Dec 27.
The cold-induced expression system in Escherichia coli is useful and we have applied this system to prepare the coelenterazine-utilizing luciferases including Renilla luciferase (RLase), a red-shifted variant of Renilla luciferase (RLase-547), the catalytic domain of Oplophorus luciferase (19kOLase) and Gaussia luciferase (GLase). The luminescence properties of the purified luciferases were characterized by using 10 kinds of C2-modified coelenterazine analogues as a substrate. The order of the maximal luminescence intensity for native coelenterazine was GLase (100%)>RLase (8.0%)>RLase-547 (0.73%)>19kOLase (0.09%) under our assay conditions. The substrate specificities of coelenterazine-utilizing luciferases for the C2-modified analogues showed significant differences, but the emission peaks catalyzed by coelenterazine-utilizing luciferases were not affected by the C2-substituted coelenterazine. These results suggest that the catalytic environment for the oxygenation process of coelenterazine and the excited species of coelenteramide might be different among coelenterazine-utilizing luciferases.
Molecular Weight Calculator Molarity Calculator Solution Dilution Calculator

Chemical Structure

CAS 55779-48-1 Coelenterazine

Quick Inquiry

Verification code

Featured Items