Calcein - CAS 1461-15-0
Molecular Formula:
Molecular Weight:
Ion Indicators and Sensors
Calcein is a fluorescent calcium indicator. It can be used for the fluorometric determination of calcium and EDTA titration of calcium.
Solid Powder
Fluorescein Complexone; Fluorexon; Oftasceine; Oftasceinum; Oftasceina; N,N'-[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl)bis(methylene)]bis[N-(carboxymethyl)-glycine
Store at -20°C
494 nm
517 nm
Canonical SMILES:
1.Fast Nongenomic Effect of Aldosterone on the Volume of Principal Cells in Collecting Tube and Genetic Heterogeneity of Epithelial Sodium Channel in the Postnatal Ontogenesis of Rat Kidney.
Logvinenko NS1, Gerbek YE2, Solenov EI2, Ivanova LN2. Bull Exp Biol Med. 2016 Mar;160(5):691-4. doi: 10.1007/s10517-016-3251-3. Epub 2016 Mar 29.
The effects of amiloride, epithelial sodium pump inhibitor, on the fast nongenomic effect of aldosterone in principal cells of an isolated segment of the distal portion of renal collecting tubes were studied in 10-day-old and adult rats. Fluorescent staining with Calcein AM showed various effects of amiloride (10(-5) M) on the stabilizing effect of aldosterone (10 nM) in hypotonic shock (280/140 mOsm/kg). Amiloride attenuated by 30% the effect of aldosterone on the amplitude of principal cell swelling in adult animals and almost completely abolished this effect in 10-day rats (p<0.05). These age-specific differences in the contribution of the distal portion of the collecting tube to the nongenomic effect of aldosterone did not depend on genetic heterogeneity of its α-subunit.
2.Microneedle-mediated intradermal delivery of epigallocatechin-3-gallate.
Puri A1, Nguyen HX1, Banga AK1. Int J Cosmet Sci. 2016 Mar 24. doi: 10.1111/ics.12320. [Epub ahead of print]
OBJECTIVE: Epigallocatechin-3-gallate (EGCG) is the physiologically most active and abundant flavanol, accounting for 50-80% of green tea catechins. It is an anti-inflammatory, anti-oxidant, chemopreventive and skin photoprotective agent. However, light sensitivity and low permeability of EGCG across the stratum corneum due to its high molecular weight as well as strong binding to the lipid bilayers in the skin makes it difficult to be used as a key ingredient in cosmetic products. The present study aimed to formulate a photostable hydrogel of EGCG with good rheological properties for dermal application and investigate the effect of skin microporation using maltose microneedles on its permeation through dermatomed porcine skin.
3.Change in viability of C2C12 myoblasts under compression, shear and oxidative challenges.
Hong Y1, Yao Y1, Wong S1, Bian L1, Mak AF2. J Biomech. 2016 Mar 18. pii: S0021-9290(16)30299-8. doi: 10.1016/j.jbiomech.2016.03.014. [Epub ahead of print]
Skeletal and epidermal loadings can damage muscle cells and contribute to the development of deep tissue injury (DTI) - a severe kind of pressure ulcers affecting many people with disability. Important predisposing factors include the multiaxial stress and strain fields in the internal tissues, particularly the vulnerable muscles around bony prominences. A careful study of the mechanical damage thresholds for muscle cell death is critical not only to the understanding of the formation of DTI, but also to the design of various body support surfaces for prevention. In this paper, we measured the mechanical damage thresholds of C2C12 myoblasts under prescribed compressive strains (15% and 30%) and shear strains (from 0% to 100%), and studied how oxidative stress, as caused potentially by reperfusion or inflammation, may affect such damage thresholds. A flat plate was used to apply a uniform compressive strain and a radially increasing shear strain on disks of Gelatin-methacrylate (GelMA) hydrogel with myoblasts encapsulated within.
4.Cellometer image cytometry as a complementary tool to flow cytometry for verifying gated cell populations.
Kuksin D1, Kuksin CA2, Qiu J1, Chan LL3. Anal Biochem. 2016 Mar 28. pii: S0003-2697(16)00110-X. doi: 10.1016/j.ab.2016.03.010. [Epub ahead of print]
Traditionally, many cell-based assays that analyze cell populations and functionalities have been performed using flow cytometry. However, flow cytometers remain relatively expensive and require highly trained operators for routine maintenance and data analysis. Recently, an image cytometry system has been developed by Nexcelom Bioscience (Lawrence, MA, USA) for automated cell concentration and viability measurement using bright-field and fluorescent imaging methods. Image cytometry is analogous to flow cytometry in that gating operations can be performed on the cell population based on size and fluorescent intensity. In addition, the image cytometer is capable of capturing bright-field and fluorescent images, allowing for the measurement of cellular size and fluorescence intensity data. In this study, we labeled a population of cells with an enzymatic vitality stain (calcein-AM) and a cell viability dye (propidium iodide) and compared the data generated by flow and image cytometry.
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CAS 1461-15-0 Calcein

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