{"id":526,"date":"2016-09-26T03:18:20","date_gmt":"2016-09-26T08:18:20","guid":{"rendered":"http:\/\/www.bocsci.com\/blog\/?p=526"},"modified":"2016-09-27T02:41:50","modified_gmt":"2016-09-27T07:41:50","slug":"identification-of-a-cdna-fragment-encoding-a-putative-selenocysteine-methyltransferase","status":"publish","type":"post","link":"https:\/\/www.bocsci.com\/blog\/identification-of-a-cdna-fragment-encoding-a-putative-selenocysteine-methyltransferase\/","title":{"rendered":"Identification of a cDNA fragment encoding a putative Selenocysteine methyltransferase"},"content":{"rendered":"

Se is incorporated into antioxidant selenoproteins, making it\u00a0an essential micronutrient for animals, microorganisms and\u00a0some other eukaryotes. In addition to the basic nutritional requirements for\u00a0Se, it has become increasingly evident that Se also has\u00a0potential health benefits. These include roles in reducing the\u00a0incidence of some debilitating disorders, such as in\u00a0improving male fertility and immune function; in reducing viral infection; and in slowing the aging process. In addition, a large body of convincing evidence has indicated that Se acts as a cancer\u00a0preventive agent when given in pharmacological amounts. It is well established that supplementation\u00a0of the diet with Se helps reduce the incidence of certain\u00a0cancers and boots the\u00a0immune system. Anticarcinogenic and\u00a0chemoprotective effects of Se have been demonstrated, too. In a long-term, double-blind study,\u00a0supplemental dietary Se was associated with significant\u00a0reductions in lung, colorectal and prostate cancer in humans. Numerous studies have demonstrated the\u00a0efficacy of methylselenocysteine<\/a> (MeSeCys) in preventing\u00a0animal mammary cancer. This non-protein seleno amino acid is\u00a0produced in certain plants including members of the Brassica and Allium genera.\u00a0While the specific mechanism for the anticancer activity of\u00a0Se has not been fully elucidated, multiple studies have\u00a0demonstrated the ability of Se to affect the cell cycle and\u00a0induce apoptosis in cancer cell line. However, excess Se can be toxic, and its bioaccumulation is toxic to wildlife.<\/p>\n

Se is biochemically similar to S, and given that the\u00a0characteristics of Se parallel those of S, it may compete with\u00a0S in many of the key enzymatic steps of the S-assimilation\u00a0pathway. The two elements are thought to be taken up and\u00a0metabolized via the same mechanisms.\u00a0Most plants contain only low foliar concentrations of Se, of\u00a0less than 25 mg\/kg dry weight, and rarely exceed 100 mg\/kg\u00a0dry weight even when grown on high-Se soils. They are\u00a0termed non-accumulators. However, a\u00a0limited number of specialized plants, which are often found\u00a0growing on soils that are naturally enriched in Se, can\u00a0accumulate high concentrations of Se in their foliage. These\u00a0accumulating plants can be divided into two groups: primary\u00a0accumulators (hyperaccumulators) and secondary accumulators (indicator species). Primary accumulators have discrimination coefficients\u00a0of\u00a0more than one, and have concentrations of Se in the range of\u00a0thousands of mg\/kg. Secondary accumulators take up Se in\u00a0proportion to the amount of Se available in the soil, they\u00a0have a DCi less than one, and tissue concentrations of Se in\u00a0the hundreds of mg\/kg. The genus\u00a0Astragalus contains the highest number of selenium (Se)-hyperaccumulating species, with some of these species\u00a0accumulating up to 0.6% of their foliar dry weight as Se.<\/p>\n

\"10236-58-5\"<\/a><\/p>\n

Structure of Seleno-L-cysteine – CAS 10236-58-5<\/a><\/h5>\n

Astragalus bisulcatus metabolizes>90% of the accumulated Se into MeSeCys in young shoot tissue. MeSeCys is\u00a0synthesized from selenocysteine<\/a> and S-methylmethionine by\u00a0the enzyme, selenocysteine methyltransferase (SMT). SMT\u00a0with the capacity to use S-methylmethionine to methylate\u00a0SeCys and produce MeSeCys has been isolated from the\u00a0Se-hyperaccumulator Astragalus bisulcatus. Heterologous expression\u00a0of AbSMT in Arabidopsis thaliana and Indian mustard increase selenium tolerance and accumulation. Recently, Sors et al. showed that SMT-like protein is present both in Se accumulating and in non-accumulating Astragalus species.\u00a0According to this knowledge, in this study, we report the\u00a0isolation of the fragment that could be the part of SMT-like\u00a0gene from the secondary accumulator Astragalus chrysochlorus. The sequence of the fragment shows a high degree\u00a0of homology (92%) with Astragalus bisulcatus SMT gene.<\/p>\n

 <\/p>\n

Reference:<\/p>\n

Sule Ar\u0131 \u2022 Ozgur Cak\u0131r \u2022 Neslihan Turgut-Kara. Acta Physiol Plant (2010) 32:1085\u20131092<\/p>\n

<\/h4>\n

Related Product\uff1a<\/h4>\n
<\/div>
<\/th><\/th><\/th><\/th><\/tr><\/thead>
CAS Number <\/td>Product Name <\/td>Molecular Weight <\/td>Molecular Formula <\/td><\/tr>
10236-58-5 <\/td>selenocysteine<\/a><\/td>168.05 <\/td>C3H7NO2Se <\/td><\/tr>
26046-90-2 <\/td>methylselenocysteine<\/a><\/td>182.08 <\/td>C4H9NO2Se <\/td><\/tr><\/tbody><\/table><\/div>\n","protected":false},"excerpt":{"rendered":"

Se is incorporated into antioxidant selenoproteins, making it\u00a0an essential micronutrient for animals, microorganisms and\u00a0some other eukaryotes. In addition to the basic nutritional requirements for\u00a0Se, it has become increasingly evident that […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[181],"tags":[316,317,315],"_links":{"self":[{"href":"https:\/\/www.bocsci.com\/blog\/wp-json\/wp\/v2\/posts\/526"}],"collection":[{"href":"https:\/\/www.bocsci.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bocsci.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bocsci.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bocsci.com\/blog\/wp-json\/wp\/v2\/comments?post=526"}],"version-history":[{"count":4,"href":"https:\/\/www.bocsci.com\/blog\/wp-json\/wp\/v2\/posts\/526\/revisions"}],"predecessor-version":[{"id":536,"href":"https:\/\/www.bocsci.com\/blog\/wp-json\/wp\/v2\/posts\/526\/revisions\/536"}],"wp:attachment":[{"href":"https:\/\/www.bocsci.com\/blog\/wp-json\/wp\/v2\/media?parent=526"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bocsci.com\/blog\/wp-json\/wp\/v2\/categories?post=526"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bocsci.com\/blog\/wp-json\/wp\/v2\/tags?post=526"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}