Anemoside B4 - CAS 129741-57-7
Catalog number: B0005-464603
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Anemoside B4 is extracted from the herb of Pulsatilla chinensis (Bge.) Regel. It inhibited the secretion of IL-10.
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B0005-464603 20mg $299 In stock
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Chinensioside A; Pulchinenoside C; Pulsatilla saponin B4
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1.Simultaneous determination of anemoside B4, phellodendrine, berberine, palmatine, obakunone, esculin, esculetin in rat plasma by UPLC-ESI-MS/MS and its application to a comparative pharmacokinetic study in normal and ulcerative colitis rats.
Yang L;Meng X;Yu X;Kuang H J Pharm Biomed Anal. 2017 Feb 5;134:43-52. doi: 10.1016/j.jpba.2016.11.021. Epub 2016 Nov 11.
A sensitive and rapid ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS/MS) method was developed for the simultaneous analysis of anemoside B4, phellodendrine, berberine, palmatine, obakunone, esculin, esculetin, toosendanin (IS;1; of anemoside B4), tetrahydropalmatine (IS;2; of phellodendrine, berberine, palmatine and obakunone) and scopoletin (IS;3; of esculin and esculetin) and to compare the pharmacokinetics of these active ingredients in normal and ulcerative colitis rats. After methanol deproteinization, solvents were evaporated at 40°C under a gentle stream of nitrogen. Chromatography was performed using a C18 column with a gradient elution of 0.1% aqueous formic acid and acetonitrile at 0.4ml/min. Detection and measurement were performed on a 4000 QTRAP UPLC-MS/MS system from AB Sciex in the multiple reaction monitoring (MRM) mode. Phellodendrine, berberine, palmatine, obakunone, esculin, esculetin, tetrahydropalmatine (IS;2;) and scopoletin (IS;3;) were monitored under positive ionization conditions. Anemoside B4, and toosendanin (IS;1;) were monitored under negative ionization conditions. The optimized mass transition ion-pairs (m/z) were 1221.
2.Role of Chinese herbal medicinal ingredients in secretion of cytokines by PCV2-induced endothelial cells.
Hu Y;He K;Wang X J Immunotoxicol. 2016;13(2):141-7. doi: 10.3109/1547691X.2015.1017624. Epub 2015 Feb 27.
While T-lymphocytes are the major cell type responsible for host responses to a virus (including induction of inflammatory responses to aid in ultimate removal of virus), other cells, including macrophages, epithelial and dendritic cells also have key roles. Endothelial cells also play important roles in physiologic/pathologic processes, like inflammation, during viral infections. As endothelial cells can be activated to release various endogenous compounds, including some cytokines, ex vivo measures of cytokine formation by the cells can be used to indirectly assess any potential endothelial dysfunction in situ. The research presented here sought to investigate potential immunolomodulatory effects of five saponins on endothelial cells: Saikosaponins A (SSA) and D (SSD), Panax Notoginseng Saponin (PNS) and Notoginsenoside R1 (SR1) and Anemoside B4 (AB4). For this, cells (porcine iliac artery endothelial line) were challenged with a virus isolate PCV2-AH for 24 h and then treated with the test saponin (at 1, 5 or 10 μg/ml) for an additional 24 h at 37 °C. The culture supernatants were then collected and analyzed for interleukin (IL)-2, -4 and -10, as well as interferon (IFN)-γ by ELISA.
3.Simultaneous determination of eight bioactive compounds by LC-MS/MS and its application to the pharmacokinetics, liver first-pass effect, liver and brain distribution of orally administrated Gouteng-Baitouweng (GB) in rats.
Tian X;Xu Z;Chen M;Hu P;Liu F;Sun Z;Liu H;Guo X;Li Z;Huang C J Chromatogr B Analyt Technol Biomed Life Sci. 2018 May 1;1084:122-131. doi: 10.1016/j.jchromb.2018.03.013. Epub 2018 Mar 9.
Only focusing on the circulating levels is insufficient for the comprehensive understanding of the physiological disposition of herbal medicine in vivo. Therefore, we conducted the comprehensive investigation on the in vivo dynamic process of orally administrated Gouteng-Baitouweng (GB), a classical herb pair with anti-Parkinson potentials. Serving as the technical base, a sensitive and selective liquid chromatography-tandem mass spectrometry method was established and validated in the plasma, liver and brain, for simultaneous determination of five alkaloids (rhynchophylline, isorhynchophylline, corynoxeine, isocorynoxeine and geissoschizine methyl ether) and three saponins (anemoside B4, anemoside A3 and 23-hydroxybetulinic acid). Following liquid-liquid extraction, favorable chromatographic behaviors of eight analytes were obtained on Waters Xbrigde C18 column within 13 min. This method elicited good linearity for the analytes at the concentration range of 0.3-1000 or 1.8-6000 ng/mL with favorable precision, accuracy and stability. Following oral administration of GB (25 g/kg) in rats, this method was applied to the quantitative analysis in the portal vein plasma, liver, systemic plasma, and brain.
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CAS 129741-57-7 Anemoside B4

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