6-Carboxytetramethylrhodamine - CAS 91809-67-5
Catalog number: B0001-100558
Molecular Formula:
Molecular Weight:
Other Fluorescent Probes
6-Carboxytetramethylrhodamine is an amine-reactive, purified single isomer, orange-fluorescent dye.
Solid Powder
540 nm
568 nm
Canonical SMILES:
1.Gene expression analysis for predicting gemcitabine resistance in human cholangiocarcinoma
Jun Sato • Takashi Kimura • Takuro Saito • Takayuki Anazawa • Akira Kenjo • Yoshihiro Sato • Takao Tsuchiya • Mitsukazu Gotoh. J Hepatobiliary Pancreat Sci (2011) 18:700–711
The mRNA levels of ribonucleotide reductase 2b (p53R2), ribonucleotide reductase 1 (RNR1), ribonucleotide reductase 2a (RNR2), dehydrocytidine kinase (dCK), human equilibrative nucleoside transporter (hENT) and multidrug resistance protein 7 (MRP7), which have an influence on the transport and metabolism of gemcitabine, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in the cancer cells were quantified by the real-time reverse transcription (RT) PCR method using an ABI PRIZM 7000 Sequence Detector System (PE Applied Biosystems, Foster City, CA, USA). Total RNA was extracted from each cell line and the gemcitabine-resistant subclones using an RNeasy Protect Mini Kit (Qiagen, Hilden, Germany) with an RNase-free DNase Set (Qiagen) according to the manufacturer’s instructions. Complementary DNA (cDNA) was produced from 1 lg of RNA using an Oligo (dT) Primer (Invitrogen, Carlsbad, CA, USA) and SuperScriptII RNase H-Reverse Transcriptase (Invitrogen). The internal probes were labeled with a reporter dye, 6-carboxyfluorescein (FAM), at the 50 end, and a quencher dye, 6-carboxytetramethylrhodamine (TAMRA), at the 30 end. The primer sequences are shown in Table 1. The sequences of CDA (Hs00156401_m1), GSTM3 (Hs00168307_m1), GSTM4 (Hs00426432_m1), and GPX3 (Hs01078670_g1) were, however, not available. The PCR reaction conditions were as follows: 50℃ for 2 min, 95℃for 10 min, and 50 cycles of 95℃ for 15 s and 60℃ for 1 min. The amounts of the PCR products were normalized to the GAPDH level.
2.Event-specific qualitative and quantitative PCR methods for the detection of genetically modified rapeseed Oxy-235
Gang Wu, Yuhua Wu, Ling Xiao, Changming Lu. Transgenic Res (2008) 17:851–862
Oligonucleotide primers and TaqMan florescent probes used in event-specific detection of Oxy-235 were designed with the aid of the software PRIMER PREMIER ver. 5.00 (PREMIER Biosoft International, Palo Alto, CA) specifying an optimal amplicon size of no more than 150 bp and a melting temperature (tm) of 60C for primers and 70C for probes. The 50-ends of the TaqMan florescent probes were labeled with the fluorescent reporter 6-carboxy fluorescein (FAM), and the 30-end was labeled with the fluorescent quencher 6-carboxytetramethylrhod amine (TAMRA).
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CAS 91809-67-5 6-Carboxytetramethylrhodamine

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