6-Carboxyfluorescein diacetate - CAS 3348-03-6
Molecular Formula:
C25H16O9
Molecular Weight:
460.39
COA:
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Application\Fluorophore:
Cell and Organelle Stains
Description:
6-Carboxyfluorescein diacetate is a membrane-permeant prodrug of 6-Carboxyfluorescein, which has been used to differentiate viable cells from apoptotic cells.
Synonyms:
6-CFDA; 3',6'-Diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylic acid
MSDS:
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InChIKey:
QMOGCCYGOPYYNT-UHFFFAOYSA-N
InChI:
InChI=1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)
Canonical SMILES:
CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=C(C=CC(=C5)C(=O)O)C(=O)O3
1.Isolation and expansion of allogeneic myeloma-specific interferon-gamma producing T cells for adoptive immunotherapy.
Ocadlikova D1, Zahradova L, Kovarova L, Penka M, Zaloudik J, Buchler T, Hajek R, Michalek J. Med Oncol. 2006;23(3):377-84.
Adoptive immunotherapy is a promising approach in the treatment of multiple myeloma. We have tested the identification, separation, and expansion of allogeneic myeloma-specific T cells in vitro. Irradiated myeloma cell line ARH 77 has been used to stimulate allogeneic CD4(+) and CD8(+) T lymphocytes. Activated myeloma-specific T cells that produced interferon-gamma were isolated using immunomagnetic beads and further expanded in vitro to numbers of up to 400 x 106 T cells. Specificity of the T lymphocytes was tested using a 5-(6-)carboxyfluoresceine diacetate succinimidyl ester (CFSE)-based cytotoxicity test. This study demonstrates the feasibility of identification and isolation of tumor-specific T cells from allogeneic donors that can be expanded in vitro to numbers useful for clinical applications.
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CAS 3348-03-6 6-Carboxyfluorescein diacetate

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