4-Methylumbelliferyl-β-D-Glucuronide - CAS 6160-80-1
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Molecular Weight:
Fluorescent Enzyme Substrates
4-Methylumbelliferyl-β-D-glucuronide is a fluorogenic substrate for β-glucuronidase. 4-Methylumbelliferyl-β-D-glucuronide is cleaved by β-glucuronidase to release the fluorescent moiety 4-methylumbelliferyl (4-MU).
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4-Methylumbelliferyl glucuronide
Store at -20°C
Melting Point:
>130°C (dec.)
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1.Variability in the characterization of total coliforms, fecal coliforms and Escherichia coli in recreational water supplies of north Mississippi, USA.
Fiello M1, Mikell AT Jr, Moore MT, Cooper CM. Bull Environ Contam Toxicol. 2014 Aug;93(2):133-7. doi: 10.1007/s00128-014-1299-1. Epub 2014 Jun 22.
The fecal coliform, Escherichia coli, is a historical organism for the detection of fecal pollution in water supplies. The presence of E. coli indicates a potential contamination of the water supply by other more hazardous human pathogens. In order to accurately determine the presence and degree of fecal contamination, it is important that standard methods approved by the US Environmental Protection Agency are designed to determine the presence of E. coli in a water supply, and distinguish E. coli from other coliform bacteria (e.g. Citrobacter, Klebsiella and Enterobacter). These genera of bacteria are present not only in fecal matter, but also in soil and runoff water and are not good indicators of fecal contamination. There is also ambiguity in determining a positive result for fecal coliforms on M-FC filters by a blue colony. When all variations of blue, including light blue or glossy blue, were examined, confirmation methods agreed with the positive M-FC result less often than when colonies that the technician would merely call "blue", with no descriptors, were examined.
2.Highly specific and rapid immuno-fluorescent visualization and detection of E. coli O104:H4 with protein-A coated magnetic beads based LST-MUG assay.
Barizuddin S1, Balakrishnan B2, Stringer RC2, Dweik M2. J Microbiol Methods. 2015 Aug;115:27-33. doi: 10.1016/j.mimet.2015.05.017. Epub 2015 May 19.
A method combining immunomagnetic separation and fluorescent sensing was developed to detect Escherichia coli (E. coli) O104:H4. The antibody specific to E. coli O104:H4 was immobilized on protein A-coated magnetic beads. This protein-A-anti E. coli O104:H4 complex was used to bind Fluorescein IsoThioCyanate (FITC) labeled E. coli O104:H4 antigen (whole cell) on it. The goal was to achieve a fluorescently detectable protein-A-anti E. coli O104:H4-E. coli O104:H4 complex on the magnetic beads. Fluorescent microscopy was used to image the magnetic beads. The resulting fluorescence on the beads was due to the FITC labeled antigen binding on the protein-A-anti E. coli O104:H4 immobilized magnetic beads. This visually proves the antigen-antibody binding. The fluorescent imaging results were obtained in 2 h if the minimum available bacteria in the sample were at least 10(5) CFU/ml. If no fluorescence was observed on the magnetic beads during fluorescent imaging, it indicates the bacterial concentration in the sample to be too low for it to have bound to the magnetic beads and hence no detection was possible.
3.Brachypodium distachyon promoters as efficient building blocks for transgenic research in maize.
Coussens G1, Aesaert S, Verelst W, Demeulenaere M, De Buck S, Njuguna E, Inzé D, Van Lijsebettens M. J Exp Bot. 2012 Jun;63(11):4263-73. doi: 10.1093/jxb/ers113. Epub 2012 Apr 20.
The biotechnological approach to improve performance or yield of crops or for engineering metabolic pathways requires the expression of a number of transgenes, each with a specific promoter to avoid induction of silencing mechanisms. In maize (Zea mays), used as a model for cereals, an efficient Agrobacterium tumefaciens-mediated transformation system has been established that is applied for translational research. In the current transformation vectors, the promoters of the 35S gene of the cauliflower mosaic virus and of the ubiquitin gene of maize are often used to drive the bialaphos-selectable marker and the transgene, respectively. To expand the number of promoters, genes with either constitutive or seed-specific expression were selected in Brachypodium distachyon, a model grass distantly related to maize. After the corresponding Brachypodium promoters had been fused to the β-glucuronidase reporter gene, their activity was followed throughout maize development and quantified in a fluorimetric assay with the 4-methylumbelliferyl β-D-glucuronide substrate.
4.Facile high-throughput forward chemical genetic screening by in situ monitoring of glucuronidase-based reporter gene expression in Arabidopsis thaliana.
Halder V1, Kombrink E1. Front Plant Sci. 2015 Jan 29;6:13. doi: 10.3389/fpls.2015.00013. eCollection 2015.
The use of biologically active small molecules to perturb biological functions holds enormous potential for investigating complex signaling networks. However, in contrast to animal systems, the search for and application of chemical tools for basic discovery in the plant sciences, generally referred to as "chemical genetics," has only recently gained momentum. In addition to cultured cells, the well-characterized, small-sized model plant Arabidopsis thaliana is suitable for cultivation in microplates, which allows employing diverse cell- or phenotype-based chemical screens. In such screens, a chemical's bioactivity is typically assessed either through scoring its impact on morphological traits or quantifying molecular attributes such as enzyme or reporter activities. Here, we describe a facile forward chemical screening methodology for intact Arabidopsis seedlings harboring the β-glucuronidase (GUS) reporter by directly quantifying GUS activity in situ with 4-methylumbelliferyl-β-D-glucuronide (4-MUG) as substrate.
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CAS 6160-80-1 4-Methylumbelliferyl-β-D-Glucuronide

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