3-Indolebutyric acid (IBA) - CAS 133-32-4
Catalog number: 133-32-4
Category: Inhibitor
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Molecular Formula:
C12H13NO2
Molecular Weight:
203.24
COA:
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Description:
3-Indolebutyric acid(IBA) is a plant hormone in the auxin family and is an ingredient in many commercial plant rooting horticultural products.
Purity:
>98%
Synonyms:
N/A
MSDS:
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InChIKey:
JTEDVYBZBROSJT-UHFFFAOYSA-N
InChI:
InChI=1S/C12H13NO2/c14-12(15)7-3-4-9-8-13-11-6-2-1-5-10(9)11/h1-2,5-6,8,13H,3-4,7H2,(H,14,15)
Canonical SMILES:
C1=CC=C2C(=C1)C(=CN2)CCCC(=O)O
1.Preparation of chitosan-modified silica nanoparticles and their applications in the separation of auxins by capillary electrophoresis.
Duan LP;Ding GS;Tang AN J Sep Sci. 2015 Nov;38(22):3976-3982. doi: 10.1002/jssc.201500810. Epub 2015 Oct 20.
In recent years, nanoparticles have gained more attention when used in separation science. In this study, chitosan-modified silica nanoparticles were successfully synthesized and characterized by transmission electron microscopy, elemental analysis and zeta potential measurements, etc. When added into the running buffer solution as pseudo-stationary phase in capillary electrophoresis, the separation of four representative auxins, i.e., indole-3-acetic acid, indole butyric acid, 2,4-dichlorophenoxyacetic acid, 1-naphthaleneacetic acid, was carried out. Some important factors, such as the nanoparticles concentration, the pH and concentration of the running buffer solution, were also investigated on the separation. Under optimized experimental conditions, all the auxins investigated can be baseline separated within 5 min with higher column performance. The method established can also be used for quantitative analysis. The relative standard deviations obtained for indole-3-acetic acid, indole butyric acid, 2,4-dichlorophenoxyacetic acid, 1-naphthaleneacetic acid were in the range of 1.6-5.7% for peak area and 0.53-1.60% for migration time. The calibration curves obtained from the peaks areas for auxins were linear in the range of 0.
2.Simultaneous analysis of different classes of phytohormones in coconut (Cocos nucifera L.) water using high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry after solid-phase extraction.
Ma Z;Ge L;Lee AS;Yong JW;Tan SN;Ong ES Anal Chim Acta. 2008 Mar 10;610(2):274-81. doi: 10.1016/j.aca.2008.01.045. Epub 2008 Jan 25.
Coconut (Cocos nucifera L.) water, which contains many uncharacterized phytohormones is extensively used as a growth promoting supplement in plant tissue culture. In this paper, a high-performance liquid chromatography (HPLC) method was developed for the simultaneous determination of various classes phytohormones, including indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), abscisic acid (ABA), gibberellic acid (GA), zeatin (Z), N(6)-benzyladenine (BA), alpha-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) in young coconut water (CW). The analysis was carried out using a reverse-phase HPLC gradient elution, with an aqueous mobile phase (containing 0.1% formic acid, pH adjusted to 3.2 with triethylamine (TEA)) modified by methanol, and solute detection made at 265 nm wavelength. The method was validated for specificity, quantification, accuracy and precision. After preconcentration of putative endogenous phytohormones in CW using C(18) solid-phase extraction (SPE) cartridges, the HPLC method was able to screen for putative endogenous phytohormones present in CW. Finally, the identities of the putative phytohormones present in CW were further confirmed using independent liquid chromatography-tandem mass spectrometry (LC-MS/MS) equipped with an electrospray ionization (ESI) interface.
3.GH3.15 acyl acid amido synthetase has a highly specific substrate preference for the auxin precursor indole-3-butyric acid.
Sherp AM;Westfall CS;Alvarez S;Jez JM J Biol Chem. 2018 Mar 23;293(12):4277-4288. doi: 10.1074/jbc.RA118.002006. Epub 2018 Feb 8.
Various phytohormones control plant growth and development and mediate biotic and abiotic stress responses. Gretchen Hagen 3 (GH3) acyl acid amido synthetases are plant enzymes that typically conjugate amino acids to indole-3-acetic acid (IAA) or jasmonic acid (JA) to inactivate or activate these phytohormones, respectively; however, the physiological and biological roles of many of these enzymes remain unclear. Using a biochemical approach, we found that the ;Arabidopsis thaliana; GH3.15 (AtGH3.15) preferentially uses indole-3-butyric acid (IBA) and glutamine as substrates. The X-ray crystal structure of the AtGH3.15·AMP complex, modeling of IBA in the active site, and biochemical analysis of site-directed mutants provide insight on active site features that lead to AtGH3.15's preference for IBA. Assay-based ;in planta; analysis of AtGH3.15-overexpressing lines indicated that their root elongation and lateral root density were resistant to IBA treatment but not to treatment with either IAA or JA. These findings suggest that AtGH3.15 may play a role in auxin homeostasis by modulating the levels of IBA for peroxisomal conversion to IAA. Analysis of ;AtGH3.15; promoter-driven yellow fluorescent protein reporter lines revealed that ;AtGH3.
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CAS 133-32-4 3-Indolebutyric acid (IBA)

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