1,4-D-Xylobiose - CAS 6860-47-5
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1.Oligosaccharides obtained by enzymatic hydrolysis of birch kraft pulp xylan: analysis by capillary zone electrophoresis and mass spectrometry.
Rydlund A1, Dahlman O. Carbohydr Res. 1997 May 12;300(2):95-102.
Neutral and acidic oligosaccharides were obtained from an unbleached birch kraft pulp by treatment with a Trichoderma reesei endoxylanase pI 9 and subsequently characterized using capillary zone electrophoresis (CZE) and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). The borate complexes of unsaturated acidic oligosaccharides having a 4-deoxy-beta-L-threo-hex-4-enopyranosyluronic acid (4 delta UA) residue linked to a beta-D-(1-->4)-xylooligosaccharide backbone were separated by CZE and detected by their UV absorption at 232 nm without prior derivatization. Pre-column derivatization with the chromophore 6-aminoquinoline (6-AQ) followed by CZE in alkaline borate buffer using detection based on absorption at 245 nm was used in the case of neutral xylosaccharides. Furthermore, MALDI-TOF-MS was employed to determine the molecular masses of both unsaturated and saturated acidic oligosaccharides.
2.Differentiation of isomeric pentose disaccharides by electrospray ionization tandem mass spectrometry and discriminant analysis.
da Costa EV1, Moreira AS, Nunes FM, Coimbra MA, Evtuguin DV, Domingues MR. Rapid Commun Mass Spectrom. 2012 Dec 30;26(24):2897-904. doi: 10.1002/rcm.6415.
RATIONALE: The structural characterization of unknown oligosaccharides remains a big challenge since a large number of isomeric structures are possible even for disaccharides. In this work, electrospray ionization collision-induced dissociation tandem mass spectrometry (ESI-CID-MS/MS) was used for the differentiation of isomeric pentose disaccharides, α-(1 → 5)-L-arabinobiose (Ara(2)) and β-(1 → 4)-D-xylobiose (Xyl(2)).
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CAS 6860-47-5 1,4-D-Xylobiose

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