Catalog number: 214262-88-1
Category: Main Product
Molecular Formula:
C9 H8 Cl F O2
Molecular Weight:
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METHYL 2-CHLORO-4-FLUOROPHENYLACETATE 98; Methyl 2-chloro-4-fluorophenylacetate 98%
Boiling Point:
240.4ºC at 760mmHg
Canonical SMILES:
1.Rhodococcus pedocola sp. nov. and Rhodococcus humicola sp. nov., two novel antibiotic-producing actinomycetes isolated from soil.
Nguyen TM1, Kim J2. Int J Syst Evol Microbiol. 2016 Mar 31. doi: 10.1099/ijsem.0.001039. [Epub ahead of print]
Two novel actinobacterial strains, UC12T and UC33T, were isolated from forest topsoil in Suwon, Gyeonggi-Do, South Korea. Comparative analysis of nearly full-length 16S rRNA sequences of UC12T and UC33T revealed close pairwise similarity with Rhodococcus species, and the UC12T and UC33T sequences were most closely related to Rhodococcus canchipurensis MBRL 353T (98.91 %) and Rhodococcus triatomae IMMIB RIV-085T (97.71 %), respectively. DNA-DNA hybridization showed 33.05-35.60 % genomic similarity between UC12T and UC33T; strain UC12T shared DNA-DNA relatedness value of 32.71-41.29 % and UC33T shared 29.12-37.91 % genomic relatedness with the closest Rhodococcus species. Both strains showed similar chemotaxonomic characteristics. The major fatty acids were C16:0, summed feature 3, C18:1ω9c, and C18:0 10-methyl. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, and phosphatidylinositol mannoside.
2.Actinomadura gamaensis sp. nov., a novel actinomycete isolated from soil in Gama, Chad.
Abagana AY1, Sun P1, Liu C1, Cao T1, Zheng W1, Zhao S1, Xiang W2,3, Wang X4. Antonie Van Leeuwenhoek. 2016 Mar 24. [Epub ahead of print]
A novel single spore-producing actinomycete, designated strain NEAU-Gz5T, was isolated from a soil sample from Gama, Chad. A polyphasic taxonomic study was carried out to establish the status of this strain. The diamino acid present in the cell wall is meso-diaminopimelic acid. Glucose, mannose and madurose occur in whole cell hydrolysates. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside and an unidentified glycolipid. The predominant menaquinones were identified as MK-9(H8) and MK-9(H6). The predominant cellular fatty acids were found to be C16:0, iso-C15:0, iso-C16:0 and C18:0 10-methyl. Phylogenetic analysis based on the 16S rRNA gene showed that strain NEAU-Gz5T belongs to the genus Actinomadura and is closely related to Actinomadura oligospora JCM 10648T (ATCC 43269T; 98.3 % similarity). However, the low level of DNA-DNA relatedness and some different phenotypic characteristics allowed the strain to be distinguished from its close relatives.
3.In Silico Designing and Analysis of Inhibitors against Target Protein Identified through Host-Pathogen Protein Interactions in Malaria.
Samant M1, Chadha N2, Tiwari AK2, Hasija Y1. Int J Med Chem. 2016;2016:2741038. doi: 10.1155/2016/2741038. Epub 2016 Jan 18.
Malaria, a life-threatening blood disease, has been a major concern in the field of healthcare. One of the severe forms of malaria is caused by the parasite Plasmodium falciparum which is initiated through protein interactions of pathogen with the host proteins. It is essential to analyse the protein-protein interactions among the host and pathogen for better understanding of the process and characterizing specific molecular mechanisms involved in pathogen persistence and survival. In this study, a complete protein-protein interaction network of human host and Plasmodium falciparum has been generated by integration of the experimental data and computationally predicting interactions using the interolog method. The interacting proteins were filtered according to their biological significance and functional roles. α-tubulin was identified as a potential protein target and inhibitors were designed against it by modification of amiprophos methyl.
4.High-speed countercurrent chromatography isolation of flavans from Ixeris chinensis and their identification by NMR spectroscopy.
Wang QH1, Bao BY1, Han JJ1, Wu RJ1. J Sep Sci. 2016 Apr 8. doi: 10.1002/jssc.201600041. [Epub ahead of print]
High-speed countercurrent chromatography, combined with macroporous resin chromatography were applied to the separation and purification of flavans from Ixeris chinensis. Four flavans, namely, 5-methoxy-7,4'-dihydroxyflavan-3-ol (1), 5,7-dimethoxy-4'-hydroxyflavan-3-ol (2), 5,7-dimethoxy-4'-hydroxyflavan (3), and 5,7-dimethoxy-8-methyl-4'-hydroxyflavan (4), were obtained from Ixeris chinensis for the first time. Their chemical structural identification was carried out by spectroscopic methods, including 1D and 2D NMR spectroscopy. Amounts of 13.2 mg of compound 1, 6.4 mg of compound 2, 5.8 mg of compound 3, and 14.5 mg of compound 4 were separated from 120 mg 75% ethanol fraction. The purities of 1-4 were 99.1, 99.2, 97.3 and 98.6 %, respectively. This article is protected by copyright. All rights reserved.
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