1.The simultaneous determination of chloride, nitrate and sulphate by isotachophoresis using bromide as a leading ion.
Prest JE1, Fielden PR. Talanta. 2008 May 15;75(3):841-5. doi: 10.1016/j.talanta.2007.12.011. Epub 2007 Dec 23.
A new method has been devised to allow the determination of small inorganic anions using isotachophoresis. This method makes use of indium(III) as a counter ion to manipulate the effective mobilities of inorganic anion species by means of complexation reactions. This new procedure successfully allowed the simultaneous determination of nitrate, chloride and sulphate to be realised on a capillary scale instrument and in a chip-based separation device. The electrolyte system developed to allow the separation to be achieved employed a 10mM bromide-based leading electrolyte containing 1.25 mM indium(III) at pH 3.15 and a terminating electrolyte of cyanoacetic acid.
2.Analysis of chloride, bromide and iodide using miniaturised isotachophoresis on a planar polymer chip.
Prest JE1, Baldock SJ, Fielden PR, Goddard NJ, Treves Brown BJ. Analyst. 2005 Oct;130(10):1375-82. Epub 2005 Aug 24.
A new method has been developed to allow the determination of the halide anions chloride, bromide and iodide using isotachophoresis. This method employs a new electrolyte system which incorporates the novel application of indium(III) as a complexing agent. This electrolyte system was devised based on the findings of an investigation into the potential for using indium(III) as a complexing counter ion to selectively manipulate the effective mobilities of halide ions. A leading electrolyte incorporating 3.5 mmol dm(-3) of indium(III) allowed the simultaneous determination of chloride, bromide and iodide to be successfully achieved. The new procedure allows such separations to be made without interference from common inorganic anions such as sulfate and nitrate. Separations were performed using a miniaturised planar poly(methyl methacrylate) chip with integrated platinum wire conductivity detection electrodes. Using this instrumentation the limits of detection were calculated to be 0.
Shen FM, Lush SF. Acta Crystallogr Sect E Struct Rep Online. 2010 Sep 15;66(Pt 10):m1260-1. doi: 10.1107/S1600536810036330.
In the title dinuclear In(III) compound, [In(2)(SO(4))(3)(C(12)H(8)N(2))(2)(H(2)O)(2)]·2H(2)O, each In(III) cation is coordinated by a 1,10-phenanthroline (phen) ligand, a water mol-ecule and three sulfate O atoms in a distorted InN(2)O(4) octa-hedral geometry. Three sulfate anions bridge two In(III) cations, forming the dinuclear entities. O-H⋯O and weak C-H⋯O hydrogen bonding is observed in the crystal structure. The crystal structure is further consolidated by π-π stacking between nearly parallel phen ring systems [dihedral angle = 4.2 (4)°], the centroid-centroid distance between benzene rings of adjacent phen ligands being 3.528 (9) Å.
4.Application of a new surface labeling reagent, EDTA derivative, on erythrocytes and platelets.
Hwang KJ, Wase AW. Biochim Biophys Acta. 1978 Sep 11;512(1):54-71.
The modes of binding of a new class of impermeant metal-chelating probe, the complex of 111In3+ to 1-(p-benzenediazonium) ethylenediamine tetraacetic acid (azo-phenyl-EDTA), to human and rabbit erythrocyte membranes and the effect of binding on the function of rabbit platelets have been studied. The metal chelate, azo-phenyl-EDTA.[111In3+] bound covalently to membrane proteins following reaction with intact erythrocytes. The amount and the pattern of labeling was assessed by sodium dodecyl sulfate (SDS)-polyacrylamide disc and slab gels for radioactivity. The pattern of labeling of intact human erythrocytes by azo-phenyl-EDTA.[111In3+], by pyridoxal phosphate-NaB3H7 and by galactose oxidase-NaB3H4 was also compared. The following results were obtained: (a) The pattern of labeling of intact human erythrocyte by azo-phenyl-EDTA.[111In3+] differed from other commonly used probes for labeling external membrane surfaces. Five polypeptides were labeled by the metal chelates.