1.Potential histidine decarboxylase inhibitors. 1. alpha- and beta-substituted histidine analogues.
DeGraw JI, Engstrom J, Ellis M, Johnson HL. J Med Chem. 1977 Dec;20(12):1671-4.
Histidine analogues with alkyl substitution at Calpha and Cbeta were prepared as potential inhibitors of specific histidine decarboxylase. Activity was assessed in vitro using extracts of rat pyloric stomach and a radioisotopic assay of 14CO2 evolved from carboxyl-14C-labeled histidine. alpha-Substituted analogues (C2-C4) including 2-hydroxyethyl were less potent than alpha-methylhistidine; the alpha-n-butyl analogue was completely inactive at 10(-3) M. Similarly, beta,beta-dimethylhistidine and homohistidine failed to exhibit activity at 10(-3) M.
2.[Expression vectors enabling synthesis of recombinant proteins as hybrids with metal-binding peptides].
Efimov VA, Fradkov AA, Kalinkina AL, Chakhmakhcheva OG. Bioorg Khim. 1995 Jan;21(1):9-16.
Plasmid vectors providing a high level expression in Escherichia coli cells of genes for heterologous polypeptides and proteins fused to peptides containing homohistidine clusters have been constructed. The obtained vectors have been used to achieve expression of genes for streptavidin, proinsulin and calcitonin. The hybrid proteins were isolated by affinity chromatography on Ni-agarose columns.