Not Intended for Therapeutic Use. For research use only.

GMBS - CAS 80307-12-6

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Category
ADCs
Product Name
GMBS
Synonyms
4-Maleimidobutyric acid N-succinimidyl ester; N-(gamma-Maleimidobutyryloxy)succinimide; 4-Maleimidobutyric acid N-hydroxysuccinimide ester;
CAS Number
80307-12-6
Description
A useful protective group in antibody drug conjugates.
Molecular Weight
280.23
Molecular Formula
C12H12N2O6
COA
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MSDS
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Canonical SMILES
C1CC(=O)N(C1=O)OC(=O)CCCN2C(=O)C=CC2=O
InChI
InChI=1S/C12H12N2O6/c15-8-3-4-9(16)13(8)7-1-2-12(19)20-14-10(17)5-6-11(14)18/h3-4H,1-2,5-7H2
InChIKey
PVGATNRYUYNBHO-UHFFFAOYSA-N
Tag
ADCs Linker
Structure
CAS 80307-12-6 GMBS
Specification
Purity
≥98%
Appearance
Soild powder
Related ADCs Linker Products
Reference Reading
1.Surface immobilization of neural adhesion molecule L1 for improving the biocompatibility of chronic neural probes: In vitro characterization.
Azemi E1, Stauffer WR, Gostock MS, Lagenaur CF, Cui XT. Acta Biomater. 2008 Sep;4(5):1208-17. doi: 10.1016/j.actbio.2008.02.028. Epub 2008 Mar 20.
Silicon-based implantable neural electrode arrays are known to experience failure during long-term recording, partially due to host tissue responses. Surface modification and immobilization of biomolecules may provide a means to improve their biocompatibility and integration within the host brain tissue. Previously, the laminin biomolecule or laminin fragments have been used to modify the neural probe's silicon surface to promote neuronal attachment and growth. Here we report the successful immobilization of the L1 biomolecule on a silicon surface. L1 is a neuronal adhesion molecule that can specifically promote neurite outgrowth and neuronal survival. Silane chemistry and the heterobifunctional coupling agent 4-maleimidobutyric acid N-hydroxysuccinimide ester (GMBS) were used to covalently bind these two biomolecules onto the surface of silicon dioxide wafers, which mimic the surface of silicon-based implantable neural probes. After covalent binding of the biomolecules, polyethylene glycol (PEG)-NH(2) was used to cap the unreacted GMBS groups.
2.Interfacial recognition of human prostate-specific antigen by immobilized monoclonal antibody: effects of solution conditions and surface chemistry.
Zhao X1, Pan F, Garcia-Gancedo L, Flewitt AJ, Ashley GM, Luo J, Lu JR. J R Soc Interface. 2012 Oct 7;9(75):2457-67. doi: 10.1098/rsif.2012.0148. Epub 2012 May 2.
The specific recognition between monoclonal antibody (anti-human prostate-specific antigen, anti-hPSA) and its antigen (human prostate-specific antigen, hPSA) has promising applications in prostate cancer diagnostics and other biosensor applications. However, because of steric constraints associated with interfacial packing and molecular orientations, the binding efficiency is often very low. In this study, spectroscopic ellipsometry and neutron reflection have been used to investigate how solution pH, salt concentration and surface chemistry affect antibody adsorption and subsequent antigen binding. The adsorbed amount of antibody was found to vary with pH and the maximum adsorption occurred between pH 5 and 6, close to the isoelectric point of the antibody. By contrast, the highest antigen binding efficiency occurred close to the neutral pH. Increasing the ionic strength reduced antibody adsorbed amount at the silica-water interface but had little effect on antigen binding.
3.High-throughput analysis of GST-fusion protein expression and activity-dependent protein interactions on GST-fusion protein arrays with a spectral surface plasmon resonance biosensor.
Jung JW1, Jung SH, Kim HS, Yuk JS, Park JB, Kim YM, Han JA, Kim PH, Ha KS. Proteomics. 2006 Feb;6(4):1110-20.
We modified gold arrays with a glutathione (GSH) surface, and investigated high-throughput protein interactions with a spectral surface plasmon resonance (SPR) biosensor. We fabricated the GSH exterior on gold surfaces by successive modification with aminoethanethiol, 4-maleimidobutyric acid N-hydroxysuccinimide ester and GSH. We immobilized GST-Rac1, GST-RhoA, the GST-Rho-binding domain of rhotekin and the GST-p21-binding domain of PAK1 onto the GSH surface, and observed specific antigen-antibody interactions on the GST-fusion protein arrays. We determined the expression of GST-fusion proteins in Escherichia coli on the GSH surface with the SPR biosensor. We then analyzed the interactions of tissue transglutaminase (tTGase), a Ca2+-dependent enzyme, with RhoA and Rac1 on the GST-fusion protein arrays with the SPR biosensor. We found that tTGase interacted with RhoA and Rac1 in a Ca2+-dependent manner, indicating that the interactions were dependent on tTGase activity.
4.Use of reconstituted influenza virus virosomes as an immunopotentiating delivery system for a peptide-based vaccine.
Pöltl-Frank F1, Zurbriggen R, Helg A, Stuart F, Robinson J, Glück R, Pluschke G. Clin Exp Immunol. 1999 Sep;117(3):496-503.
Immunopotentiating reconstituted influenza virosomes (IRIV) were used as a delivery system for the synthetic peptide-based malaria vaccine SPf66. The reduced SPf66 peptide molecules containing terminal cysteine residues were covalently attached to phosphatidylethanolamine with the heterobifunctional crosslinker gamma-maleimidobutyric acid N-hydroxysuccinimide ester. The SPf66-phosphatidylethanolamine was incorporated into IRIV and BALB/c mice were immunized twice by intramuscular injection with peptide-loaded virosomes. Titres of elicited anti-SPf66 IgG were determined by ELISA. These titres were significantly higher and the required doses of antigen were lower, when mice had been preimmunized with a commercial whole virus influenza vaccine. After preimmunization with the influenza vaccine, SPf66-IRIV elicited far more consistently anti-SPf66 antibody responses than SPf(66)n adsorbed to alum. MoAb produced by four B cell hybridoma clones derived from a SPf66-IRIV-immunized mouse cross-reacted with Plasmodium falciparum blood stage parasites in immunofluorescence assays.
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