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Fluorescent labeling of proteins

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Protein, antibodies, peptides, nucleic acids, ligands, synthetic oligonucleotides and other biomolecules, labeling with fluorescent molecules that allows the sensing and visualizing of protein dynamics, localization, and protein-protein interactions, is an invaluable technique to understand protein functions and networks in living cells.
Various labeling techniques are developed in BOC Sciences such as enzymatic labeling, protein labeling, genetic labeling and quantum dot are widely utilized to meet our customers’ need.

Please find our labeling methods for tracking biomolecules as below:

1. Enzymatic labeling

Incorporate functionalities into proteins at specific sites via enzymatic reactions is one of the most important approaches for protein labeling. The incorporated tags then enable reactions that are chemoselective, whose functional groups not only are inert in biological media, but also do not occur natively in proteins or other macromolecules.

2. Chemical labeling

Chemical labeling or the use of chemical tags could offer a wider range of colors and photo chemical properties. Also, chemical tags are preferred over fluorescent proteins due to the architectural and size limitations of the fluorescent protein's characteristic b-barrel.

3. Protein labeling

A short tag is used to minimize disruption of protein folding and function in protein labeling. Sometimes, transition metals are also used to link specific residues in the tags to site-specific targets such as the N, C-termini, or internal sites within the protein. 

4. Genetic labeling

Genetic labeling, also known as chromosome painting, is a genetic labeling technique utilizes probes that are specific for chromosomal sites along the length of a chromosome. This technique allows abnormalities such as deletions and duplications to be revealed.

5. Quantum dot labeling

The tunable absorption spectrum and high extinction coefficients of quantum dots have made them attractive for light harvesting technologies such as labeling.

With the proliferation of fluorescent proteins in recent years, there are many fluorescent protein combinations that can be imaged together. Genetic encoding of fluorescent proteins (FPs) such as Ethidium bromide, fluorescein and green fluorescent protein are the most popular technique for protein labeling because of some important advantages, including high labeling specificity and simplicity. BOC Sciences offers a full spectrum of labeling kits to prepare conjugates with enzymes, fluorophore, biotin as well as use with immunochemistry, fluorescence in situ hybridization (FISH), cell tracing and receptor labeling.

Here is the products and services that we provide:

• Offer different kind of fluorescent reagents, labeling Kits;

Name Quantity Ex(nm) Em(nm) Shelf life Lead time
RTGreen nucleic acid gel stain *20× concentrate in water* 500 μL 495 520 12 months 1 week
RTGreen nucleic acid gel stain *20× concentrate in water* 1 mL 495 520 12 months 1 week
2× SYBR Green qPCR Mix 50x 50μL 497 525 6 months 1 week
2× SYBR Green qPCR Mix 200x 50μL 497 525 6 months 1 week
ROX Reference Dye 50 μL 568 591 12 months 1 week
ROX Reference Dye 200 μL 568 591 12 months 1 week
PicaGreen dsDNA Assay Kit *2000 assays* 10×100 μL  488 520 12months 1 week
PicaGreen dsDNA Assay Kit *2000 assays* 1 mL 488 520 12months 1 week
OligoGreen ssDNA Assay Kit *2000 assays* 10×100 μL 488 520 12months 1 week
OligoGreen ssDNA Assay Kit *2000 assays* 1 mL 488 520 12months 1 week
RibaGreen RNA Assay Kit *2000 assays* 10×100 μL  488 520 12months 1 week
RibaGreen RNA Assay Kit *2000 assays* 1 mL 488 520 12months 1 week

  • • Call or email us the chemical (small molecular, peptide, protein or antibody) that need labeling;
  • • Evaluating the amount of the fluorescent reagent and the condition that will be used. Make the project plan according to customer’s request and Quote;
  • • Report on the progress of the labeling, usually it will be finished in 3-5 days;
  • • Delivery of the labeled product together with Project report, including HPLC, NMR, MS etc.;
  • • Pay after receiving the product.

 

With advanced technology and rich experience, we will offer you the best service in this area. Please do not hesitate to contact us and let us know your need.

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