1.Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage.
Wu LM1, Guo R, Hui L, Ye YG, Xiang JM, Wan CY, Zou M, Ma R, Sun XZ, Yang SJ, Guo DZ. J Vet Sci. 2014 Dec;15(4):475-83. Epub 2014 Jun 20.
Chronic enteritis can produce an excess of reactive oxygen species resulting in cellular damage. Stanniocalcin-1(STC-1) reportedly possesses anti-oxidative activity, the aim of this study was to define more clearly the direct contribution of STC-1 to anti-oxidative stress in cattle. In this study, primary intestinal epithelial cells (IECs) were exposed to hydrogen peroxide (H2O2) for different time intervals to mimic chronic enteritis-induced cellular damage. Prior to treatment with 200 µM H2O2, the cells were transfected with a recombinant plasmid for 48 h to over-express STC-1. Acridine orange/ ethidium bromide (AO/EB) double staining and trypan blue exclusion assays were then performed to measure cell viability and apoptosis of the cells, respectively. The expression of STC-1 and apoptosis-related proteins in the cells was monitored by real-time PCR and Western blotting. The results indicated that both STC-1 mRNA and protein expression levels positively correlated with the duration of H2O2 treatment.
2.Bioinspired synthesis of fluorescent calcium carbonate/carbon dot hybrid composites.
Guo S1, Yang M, Chen M, Zhang J, Liu K, Ye L, Gu W. Dalton Trans. 2015 May 7;44(17):8232-7. doi: 10.1039/c5dt00837a.
Herein, we report a novel method to synthesise fluorescent calcium carbonate/carbon dots (CaCO3/CDs) by simply mixing CaCl2 and Na2CO3 solutions in the presence of CDs. There are two roles of CDs in this easy and cost-effective biomimetic strategy, that is as the template to direct the formation and assembly of calcite nanocrystals into hierarchical spheres with diameters in the range of 200-300 nm and simultaneously as the phosphor to enable the CaCO3 to emit blue fluorescence under UV (365 nm) irradiation with a quantum yield of 56.2%. The CaCO3/CD hybrid composites possessing unique fluorescence properties are potentially useful in various applications.
3.Combined histological and hematological assessment of iron-induced organ damage in a gerbil model of iron overload.
Wang M1, Liu RR1, Wang CJ2, Kang W3, Yang GH1, Zhong WN3, Lai YR1. Am J Transl Res. 2015 Feb 15;7(2):385-92. eCollection 2015.
BACKGROUND: Previous studies with gerbil models have suggested that excessive iron exposure causes cardiomyopathy and hepatic injury, but pathological analysis was not comprehensive, preventing a detailed understanding of how the metal induces this damage.
4.Ultrafast light induced unusually broad transient absorption in the sub-bandgap region of GeSe2 thin film.
Barik AR1, Bapna M1, Drabold DA2, Adarsh KV1. Sci Rep. 2014 Jan 14;4:3686. doi: 10.1038/srep03686.
In this paper, we show for the first time that ultrafast light illumination can induce an unusually broad transient optical absorption (TA), spanning of ≈ 200 nm in the sub-bandgap region of chalcogenide GeSe2 thin films, which we interpret as being a manifestation of creation and annihilation of light induced defects. Further, TA in ultrashort time scales show a maximum at longer wavelength, however blue shifts as time evolves, which provides the first direct evidence of the multiple decay mechanisms of these defects. Detailed global analysis of the kinetic data clearly demonstrates that two and three decay constants are required to quantitatively model the experimental data at ps and ns respectively.