1.Sodium d-fructose-1,6-diphosphate vs. sodium monohydrogen phosphate in total parenteral nutrition: a comparative in vitro assessment of calcium phosphate compatibility.
Prinzivalli M1, Ceccarelli S. JPEN J Parenter Enteral Nutr. 1999 Nov-Dec;23(6):326-32.
BACKGROUND: The supply of high amounts of calcium (Ca) and phosphorus (P) during total parenteral nutrition (TPN) is matter of concern because of the risk associated with calcium phosphate precipitation. The in vitro Ca-P compatibility in ready-for-use TPN solutions after the addition of different concentrations of inorganic phosphate or d-fructose-1,6-diphosphate (FDP) and calcium chloride was evaluated.
2.4-hydroxy-2,5-dimethyl-3(2H)-furanone formation by Zygosaccharomyces rouxii: effect of the medium.
Hauck T1, Brühlmann F, Schwab W. J Agric Food Chem. 2003 Jul 30;51(16):4753-6.
The formation of 4-hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF) by Zygosaccharomyces rouxii was studied in yeast-peptone-dextrose medium containing d-fructose 1,6-diphosphate under various culture conditions. Cell growth and HDMF production was heavily dependent on medium pH and sodium chloride concentration. Higher pH values of the nutrient medium had a positive effect on HDMF formation but retarded cell growth resulting in an optimal pH value of 5.1 with regard to the yield of HDMF. Salt stress stimulated HDMF formation by Z. rouxii as increasing sodium chloride concentration led to higher amounts of HDMF. The HDMF concentration in the culture supernatant and HDMF formation per yeast cell peaked at 20% sodium chloride in the nutrient medium. The nonutilizable carbohydrate d-xylose displayed a weak effect on HDMF formation, and the addition of glycerol to salt-stressed cells had no effect on the production of HDMF.