1.Development of lamellar gel phase emulsion containing marigold oil (Calendula officinalis) as a potential modern wound dressing.
Okuma CH1, Andrade TA2, Caetano GF2, Finci LI3, Maciel NR4, Topan JF4, Cefali LC5, Polizello AC6, Carlo T7, Rogerio AP8, Spadaro AC6, Isaac VL5, Frade MA2, Rocha-Filho PA4. Eur J Pharm Sci. 2015 Apr 25;71:62-72. doi: 10.1016/j.ejps.2015.01.016. Epub 2015 Feb 12.
Appropriate therapeutics for wound treatments can be achieved by studying the pathophysiology of tissue repair. Here we develop formulations of lamellar gel phase (LGP) emulsions containing marigold (Calendula officinalis) oil, evaluating their stability and activity on experimental wound healing in rats. LGP emulsions were developed and evaluated based on a phase ternary diagram to select the best LGP emulsion, having a good amount of anisotropic structure and stability. The selected LGP formulation was analyzed according to the intrinsic and accelerated physical stability at different temperatures. In addition, in vitro and in vivo studies were carried out on wound healing rats as a model. The LGP emulsion (15.0% marigold oil; 10.0% of blend surfactants and 75.0% of purified water [w/w/w]) demonstrated good stability and high viscosity, suggesting longer contact of the formulation with the wound. No cytotoxic activity (50-1000 μg/mL) was observed in marigold oil.
2.Bio-active nanoemulsions enriched with gold nanoparticle, marigold extracts and lipoic acid: In vitro investigations.
Guler E1, Barlas FB1, Yavuz M2, Demir B1, Gumus ZP3, Baspinar Y4, Coskunol H5, Timur S6. Colloids Surf B Biointerfaces. 2014 Sep 1;121:299-306. doi: 10.1016/j.colsurfb.2014.05.026. Epub 2014 Jun 23.
A novel and efficient approach for the preparation of enriched herbal formulations was described and their potential applications including wound healing and antioxidant activity (cell based and cell free) were investigated via in vitro cell culture studies. Nigella sativa oil was enriched with Calendula officinalis extract and lipoic acid capped gold nanoparticles (AuNP-LA) using nanoemulsion systems. The combination of these bio-active compounds was used to design oil in water (O/W) and water in oil (W/O) emulsions. The resulted emulsions were characterized by particle size measurements. The phenolic content of each nanoemulsion was examined by using both colorimetric assay and chromatographic analyses. Two different methods containing cell free chemical assay (1-diphenyl-2-picrylhydrazyl method) and cell based antioxidant activity test were used to evaluate the antioxidant capacities. In order to investigate the bio-activities of the herbal formulations, in vitro cell culture experiments, including cytotoxicity, scratch assay, antioxidant activity and cell proliferation were carried out using Vero cell line as a model cell line.
3.Herbal infusions of black seed and wheat germ oil: Their chemical profiles, in vitro bio-investigations and effective formulations as Phyto-Nanoemulsions.
Gumus ZP1, Guler E2, Demir B3, Barlas FB3, Yavuz M4, Colpankan D3, Senisik AM5, Teksoz S6, Unak P6, Coskunol H7, Timur S8. Colloids Surf B Biointerfaces. 2015 Sep 1;133:73-80. doi: 10.1016/j.colsurfb.2015.05.044. Epub 2015 Jun 4.
The reported studies related to black seed oil (BSO) and wheat germ oil (WGO) have illustrated that they have a wide range of biological activities. Therefore, enhancing the amount of bio-active compounds that caused higher cell based anti-oxidative effect as well as cell proliferation, etc. in seed oils, infusion of crude plant material has been gained importance as a traditional technique. Herein, we accomplished the infusion of Calendula flowers that also contains many phyto-constituents into BSO and WGO. After the infusion of oils, the change of phytochemical amount was investigated and evaluated according to the oils by chromatography, radical scavenging activity. Subsequently, for investigating the biological impact upon live cells, cytotoxicity, cell-based antioxidant capacity, wound healing and radioprotective activity were tested with monkey kidney fibroblast like cells (Vero) and HaCaT keratinocytes. In vitro cell based experiments (wound healing and radioprotective activity) confirmed that Calendula infused BSO and WGO have greater bio-activity when compared to those plain forms.