Calcium D-Galactonate - CAS 69617-74-9
Catalog number: 69617-74-9
Category: Main Product
Molecular Formula:
C6H11O7.xCa
Molecular Weight:
520.45
COA:
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Purity:
95%
Synonyms:
D-GALACTONIC ACID CALCIUM SALT PENTAHYDRATE; CALCIUM-D-GALACTONATE PENTAHYDRATE; Calium-D-galactonate pentahydrate
MSDS:
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1.Purification and characterization of a novel lactonohydrolase, catalyzing the hydrolysis of aldonate lactones and aromatic lactones, from Fusarium oxysporum.
Shimizu S1, Kataoka M, Shimizu K, Hirakata M, Sakamoto K, Yamada H. Eur J Biochem. 1992 Oct 1;209(1):383-90.
A novel lactonohydrolase, an enzyme that catalyzes the hydrolysis of aldonate lactones to the corresponding aldonic acids, was purified 10-fold to apparent homogeneity, with a 61% overall recovery, from Fusarium oxysporum AKU 3702, through a purification procedure comprising DEAE-Sephacel, octyl-Sepharose CL-4B and hydroxyapatite chromatographies and crystallization. The molecular mass of the native enzyme, as estimated by high-performance gel-permeation chromatography, is 125 kDa, and the subunit molecular mass is 60 kDa. The enzyme contains 15.4% (by mass) glucose equivalent of carbohydrate, and about 1 mol calcium/subunit. The enzyme hydrolyzes aldonate lactones, such as D-galactono-gamma-lactone and L-mannono-gamma-lactone, stereospecifically. Furthermore, it can catalyze the asymmetric hydrolysis of D-pantoyl lactone, which is a promising chiral building block for the chemical synthesis of D-pantothenate. These reactions are reversible, and the reaction equilibrium at pH 6.
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CAS 69617-74-9 Calcium D-Galactonate

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