CAGED FLUORESCEIN MALEIMIDE* - CAS 167776-24-1
Catalog number: 167776-24-1
Category: Main Product
Molecular Formula:
C37H26N4O12
Molecular Weight:
718.628
COA:
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Purity:
95%
Synonyms:
o-{[n-(maleimido-methylcarbonyl)-n-carboxymethylamino]-3-aza-2-propenyl}-o'-(2-nitrobenzyl)fluorescein
Storage:
-20ºC
MSDS:
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Quantity:
Data not available, please inquire.
InChIKey:
ZCVMMLGSUDDYSY-DVRIZHICSA-N
InChI:
InChI=1S/C37H26N4O12/c42-23-9-11-27-30(17-23)52-31-18-24(10-12-28(31)37(27)26-7-3-2-6-25(26)36(47)53-37)50-16-15-38-40(34(45)19-39-32(43)13-14-33(39)44)20-35(46)51-21-22-5-1-4-8-29(22)41(48)49/h1-15,17-18,42H,16,19-21H2/b38-15+
Canonical SMILES:
C1=CC=C(C(=C1)COC(=O)CN(C(=O)CN2C(=O)C=CC2=O)N=CCOC3=CC4=C(C=C3)C5(C6=C(O4)C=C(C=C6)O)C7=CC=CC=C7C(=O)O5)[N+](=O)[O-]
1.Caged protein conjugates and light-directed generation of protein activity: preparation, photoactivation, and spectroscopic characterization of caged G-actin conjugates.
Marriott G1. Biochemistry. 1994 Aug 9;33(31):9092-7.
A simple method is described to prepare caged (inactive) protein complexes using the amino group-directed photo-deprotection group [(nitroveratryl)oxy]chlorocarbamate (NVOC-Cl). In this study, I show how the polymerization activity of G-actin in physiological salt solution is lost upon conjugation of essential lysine residues of G-actin with NVOC-Cl. Reaction conditions were optimized to prepare caged G-actin in high yield, and the conjugate was characterized by biochemical and absorption spectroscopic methods. Upon excitation of caged G-actin in physiological salt solutions with near-ultraviolet light, an efficient photo-deprotection reaction occurs via photoisomerization of the (nitrophenyl)ethyl group of NVOC, which results in cleavage of the carbamate linkage between the protection reagent and G-actin. A standard irradiation condition was then defined which leads to photoactivation of F-actin from caged G-actin with a yield of more than 90%.
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CAS 167776-24-1 CAGED FLUORESCEIN MALEIMIDE*

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