1.Genome wide characterization of short tandem repeat markers in sweet orange (Citrus sinensis).
Biswas MK1, Xu Q1, Mayer C2, Deng X1. PLoS One. 2014 Aug 22;9(8):e104182. doi: 10.1371/journal.pone.0104182. eCollection 2014.
Sweet orange (Citrus sinensis) is one of the major cultivated and most-consumed citrus species. With the goal of enhancing the genomic resources in citrus, we surveyed, developed and characterized microsatellite markers in the ≈347 Mb sequence assembly of the sweet orange genome. A total of 50,846 SSRs were identified with a frequency of 146.4 SSRs/Mbp. Dinucleotide repeats are the most frequent repeat class and the highest density of SSRs was found in chromosome 4. SSRs are non-randomly distributed in the genome and most of the SSRs (62.02%) are located in the intergenic regions. We found that AT-rich SSRs are more frequent than GC-rich SSRs. A total number of 21,248 SSR primers were successfully developed, which represents 89 SSR markers per Mb of the genome. A subset of 950 developed SSR primer pairs were synthesized and tested by wet lab experiments on a set of 16 citrus accessions. In total we identified 534 (56.21%) polymorphic SSR markers that will be useful in citrus improvement.
2.Nutritional and sensory evaluation of a complementary food formulated from rice, faba beans, sweet potato flour, and peanut oil.
Mahmoud AH, El Anany AM. Food Nutr Bull. 2014 Dec;35(4):403-13.
BACKGROUND: Childhood malnutrition is a common disorder in developing countries.
3.Topical tranexamic Acid does not affect electrophysiologic or neurovascular sciatic nerve markers in an animal model.
Schwarzkopf R1, Dang P, Luu M, Mozaffar T, Gupta R. Clin Orthop Relat Res. 2015 Mar;473(3):1074-82. doi: 10.1007/s11999-014-4098-4. Epub 2015 Jan 6.
BACKGROUND: Tranexamic acid is a safe and effective antifibrinolytic agent used systemically and topically to reduce blood loss and transfusion rate in patients having TKA or THA. As the hip does not have a defined capsule, topical application of tranexamic acid may entirely envelop the sciatic nerve during THA. Accidental application of tranexamic acid onto the spinal cord in spinal anesthesia has been shown to produce seizures; therefore, we sought to investigate if topical application of tranexamic acid on the sciatic nerve has a deleterious effect.
4.Identification of the pharmacophore of the CC chemokine-binding proteins Evasin-1 and -4 using phage display.
Bonvin P1, Dunn SM2, Rousseau F3, Dyer DP4, Shaw J2, Power CA2, Handel TM4, Proudfoot AE5. J Biol Chem. 2014 Nov 14;289(46):31846-55. doi: 10.1074/jbc.M114.599233. Epub 2014 Sep 29.
To elucidate the ligand-binding surface of the CC chemokine-binding proteins Evasin-1 and Evasin-4, produced by the tick Rhipicephalus sanguineus, we sought to identify the key determinants responsible for their different chemokine selectivities by expressing Evasin mutants using phage display. We first designed alanine mutants based on the Evasin-1·CCL3 complex structure and an in silico model of Evasin-4 bound to CCL3. The mutants were displayed on M13 phage particles, and binding to chemokine was assessed by ELISA. Selected variants were then produced as purified proteins and characterized by surface plasmon resonance analysis and inhibition of chemotaxis. The method was validated by confirming the importance of Phe-14 and Trp-89 to the inhibitory properties of Evasin-1 and led to the identification of a third crucial residue, Asn-88. Two amino acids, Glu-16 and Tyr-19, were identified as key residues for binding and inhibition of Evasin-4.