HOME > Drug Discovery > In Vitro Assay > ADME Profiling

In vitro ADMET assays could be conducted to evaluate and improve metabolism, bioavailability, pharmacology and toxicology of compounds.

Early stage ADMET testing, integrated with Medicinal Chemistry and structure optimization programs, typically include:

Aqueous solubility under physiological conditions
Metabolic and chemical stability
Membrane permeability (PAMPA, Caco-2, etc.)
Inhibition of major metabolic enzymes (CYP450s)

Additional ADMET studies include numerous analytical services, as well as:

Metabolite profiling
Metabolite mass balance and isolation from urine, blood, feces, etc.
Metabolite structure elucidation
Metabolite synthesis
Animal species selection
CYP phenotyping
Induction of major CYP isoforms
Enhanced PAMPA & cell-based permeability models
Plasma protein binding
Cytotoxicity assays against human cell lines
Reactive metabolite evaluation
Time-dependent inhibition of CYP isoforms
Ames Tests for mutagenicity
Tissue and biological fluid extractions
Bioanalytical Method Development
Bioanalytical Studies