1.Simultaneous quantification of 2',3',5'-tri-O-acetyl-N6-(3-hydroxylaniline)adenosine and its principal metabolites in hamster blood by LC-MS/MS and its application in pharmacokinetics study.
Jia Y1, Wang B2, Wu X2, Li S2, Hu J2, Wang D3, Zhu H4, Li Y5. J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Apr 2;1022:46-53. doi: 10.1016/j.jchromb.2016.04.004. [Epub ahead of print]
2',3',5'-Tri-O-acetyl-N6-(3-hydroxylaniline)adenosine (IMM-H007, once called WS070117) is being developed as a novel anti-hyperlipidemia agent for its high efficacy and low toxicity. In this study, a sensitive and specific liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was established for the simultaneous quantification of IMM-H007 and its two major metabolites (3S,4R,5R)-2-(hydroxymethyl)-5-(6-((3-hydroxyphenyl)amino)-9H-purin-9-yl)tetrahdrofuran-3,4-diol (M1) and ((2R,3S,4R,5R)-3,4-dihydroxy-5-(6-((3-hydroxyphenyl)amino)-9H-purin-9-yl)tetrahydrofuran-2-yl)methyl dihydrogen phosphate (MP) in hamster blood. An analogue of IMM-H007, WS070119 was used as the internal standard. Blood samples were prepared by a simple protein precipitation with acetonitrile. The chromatographic separation was performed on a ReproSil-Pur 120C18 column (3μm, 2mm×100mm) with a gradient mobile phase of methanol/water containing 0.1% formic acid (v/v) in a flow rate of 0.
2.FTY720 inhibits germ cell apoptosis in testicular torsion/detorsion.
Shih HJ1, Yen JC2, Chiu AW3, Chow YC4, Pan WH2, Huang CJ5. J Surg Res. 2016 May 1;202(1):155-64. doi: 10.1016/j.jss.2015.12.035. Epub 2015 Dec 30.
BACKGROUND: Testicular torsion/detorsion (T/D) can induce germ cells apoptosis, which may lead to impairment of spermatogenesis. FTY720, an agonist of the sphingosine-1-phosphate receptor 1 (S1PR1), inhibits apoptosis in ischemic stroke. We examined whether FTY720 could mitigate germ cell apoptosis in testicular T/D rats.
3.New approach for the strategic control of gastrointestinal nematodes in grazed beef cattle during the growing phase in central Brazil.
Heckler RP1, Borges DG2, Vieira MC2, Conde MH2, Green M2, Amorim ML2, Echeverria JT2, Oliveira TL2, Moro E3, Van Onselen VJ2, Borges FA4. Vet Parasitol. 2016 May 15;221:123-9. doi: 10.1016/j.vetpar.2016.03.010. Epub 2016 Mar 18.
We evaluated the effect of different treatment protocols against gastrointestinal nematodes in Nelore beef cattle during the growing phase in the municipality of Terenos, MS, in central Brazil from May 2013 to April 2014 and from May 2014 to April 2015. Ninety-six Nelore calves were kept on Brachiaria brizantha grass during each trial period and were distributed into six experimental groups (replicate paddocks for each group) based on live weight and the number of eggs per gram of feces (EPG): T1 (control)-treated in May, July and September with a saline solution; T2-treated in May and November with 700μg/kg doramectin; T3-treated in May (doramectin), July (4.7mg/kg levamisole phosphate) and September (doramectin); T4-treated in May (doramectin), July (200μg/kg moxidectin) and September (doramectin); T5-treated in May (doramectin), August (levamisole phosphate) and November (doramectin) and T6-treated in May (doramectin), August (moxidectin) and November (doramectin).
4.Characterization of RNA isolated from eighteen different human tissues: results from a rapid human autopsy program.
Walker DG1,2, Whetzel AM3, Serrano G3, Sue LI3, Lue LF4, Beach TG3. Cell Tissue Bank. 2016 Apr 16. [Epub ahead of print]
Many factors affect the integrity of messenger RNA from human autopsy tissues including postmortem interval (PMI) between death and tissue preservation and the pre-mortem agonal and disease states. In this communication, we describe RNA isolation and characterization of 389 samples from 18 different tissues from elderly donors who were participants in a rapid whole-body autopsy program located in Sun City, Arizona ( www.brainandbodydonationprogram.org ). Most tissues were collected within a PMI of 2-6 h (median 3.15 h; N = 455), but for this study, tissue from cases with longer PMIs (1.25-29.25 h) were included. RNA quality was assessed by RNA integrity number (RIN) and total yield (ng RNA/mg tissue). RIN correlated with PMI for heart (r = -0.531, p = 0.009) and liver (r = -558, p = 0.0017), while RNA yield correlated with PMI for colon (r = -485, p = 0.016) and skin (r = -0.460, p = 0.031). RNAs with the lowest integrity were from skin and cervix where 22.