1.Stereospecific ligands and their complexes. XXII. Synthesis and antitumor activity of palladium(II) complexes with some esters of (S,S)-ethylenediamine-N,N'-di-(2,2'-di(4-hydroxy-benzyl))-acetic acid.
Stojković DLj1, Jevtić VV2, Radić GP3, Todorović DV3, Petrović M4, Zarić M5, Nikolić I5, Baskić D3, Trifunović SR1. J Inorg Biochem. 2015 Feb;143:111-6. doi: 10.1016/j.jinorgbio.2014.12.001. Epub 2014 Dec 10.
Four new ligands and their palladium(II) complexes of general formula R2-S,S-eddtyr (L1-L4) and [PdCl2(R2-S,S-eddtyr)] (C1-C4) (R=ethyl, n-propyl, n-butyl and n-pentyl; S,S-eddtyr·2HCl=ethylenediamine-N,N'-di-(2,2'-di(4-hydroxy-benzyl))-acetic acid dihydrochloride have been synthesized and characterized by microanalysis, infrared, (1)H and (13)C NMR spectroscopy. Cytotoxicity for ligands and complexes on two different cell lines (human breast cancer, MDA-MB-231 and human lung cancer, A549 cell lines) and human chronic lymphocytic leukemia cells (CLL) was investigated using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay.
2.Targeting CD44 expressing cancer cells with anti-CD44 monoclonal antibody improves cellular uptake and antitumor efficacy of liposomal doxorubicin.
Arabi L1, Badiee A2, Mosaffa F3, Jaafari MR4. J Control Release. 2015 Dec 28;220(Pt A):275-86. doi: 10.1016/j.jconrel.2015.10.044. Epub 2015 Oct 27.
Although liposomes improve the safety and pharmacokinetic properties of free drugs, they have not sufficiently enhanced the therapeutic efficacy compared to them. To address this problem, targeted therapy of tumor cells holds great promise to further enhance therapeutic index and decreases off-target effects compared with non-targeted liposomes. In the context of antibody-mediated targeted cancer therapy, we evaluated the anti-tumor activity and therapeutic efficacy of Doxil, and that of Doxil modified with a monoclonal antibody (mAb) against CD44, which is one of the most well-known surface markers associated with Cancer Stem Cells (CSCs). Flow cytometry analyses and confocal laser scanning microscopy results showed significant enhanced cellular uptake of CD44-targeted Doxil (CD44-Doxil) in CD44-positive C-26 cells compared to Doxil. However, CD44-negative NIH-3T3 cells showed a similar uptake and in vitro cytotoxicity with both CD44-Doxil and non-targeted Doxil.
3.Enhanced peroxidase-like activity of porphyrin functionalized ceria nanorods for sensitive and selective colorimetric detection of glucose.
Liu Q1, Ding Y2, Yang Y2, Zhang L2, Sun L2, Chen P2, Gao C2. Mater Sci Eng C Mater Biol Appl. 2016 Feb 1;59:445-53. doi: 10.1016/j.msec.2015.10.046. Epub 2015 Oct 17.
Ceria nanorods modified with 5,10,15,20-tetrakis(4-carboxyl phenyl)-porphyrin (H2TCPP) were prepared. These nanocomposites (H2TCPP-CeO2) exhibited the intrinsic peroxidase-like activity and could catalyze the oxidation of classical peroxidase substrate 3,3',5,5'-tetramethylbiphenyl dihydrochloride (TMB·2HCl) in the presence of H2O2 to produce a typical color reaction from colorless to blue. Our results demonstrated that both the H2TCPP-CeO2 nanocomposites and CeO2 nanorods exhibited higher thermal durance than that of HRP. The affinity of The H2TCPP-CeO2 nanocomposites toward H2O2 and TMB is similar to that of HRP. Fluorescent results indicated that the catalytic mechanism of the H2TCPP-CeO2 nanocomposites were from the decomposition of H2O2 into hydroxyl radicals. Based on these studies, a simple, sensitive, and selective visual and colorimetric method using TMB as the substrate was designed to detect glucose when combined with glucose oxidase.
4.Prevention of vascular smooth muscle cell proliferation and injury-induced neointimal hyperplasia by CREB-mediated p21 induction: An insight from a plant polyphenol.
Sun L1, Zhao R1, Zhang L1, Zhang W2, He G1, Yang S1, Song J1, Du G3. Biochem Pharmacol. 2016 Mar 1;103:40-52. doi: 10.1016/j.bcp.2016.01.015. Epub 2016 Jan 22.
Cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA)/cAMP response element (CRE)-binding protein (CREB) signaling cascade negatively regulates platelet-derived growth factor BB (PDGF-BB)-induced smooth muscle cell (SMC) proliferation, which is a critical event in the initiation and development of restenosis and atherosclerotic lesions. Salvianolic acid A (SAA) is one of the most abundant polyphenols extracted from salvia. The aim of this study is to investigate whether SAA exerts an action on PDGF-BB-induced proliferation via cAMP/PKA/CREB mechanism. SAA blunts PDGF-BB-induced human umbilical artery smooth muscle cell (hUASMC) proliferation via p21 induction, as evidenced by its increased mRNA and protein expression levels. The SAA-induced upregulation of p21 involves the cAMP/PKA signaling pathway; a cAMP analog mimicked the effects of SAA and a specific cAMP/PKA inhibitor opposed these effects. SAA also activated CREB, including phosphorylation at Ser133, and induced its nuclear translocation.