1.Intraoperative Detection of Superficial Liver Tumors by Fluorescence Imaging Using Indocyanine Green and 5-aminolevulinic Acid.
Kaibori M1, Matsui K2, Ishizaki M2, Iida H2, Okumura T2, Sakaguchi T2, Inoue K2, Ikeura T3, Asano H4, Kon M2. Anticancer Res. 2016 Apr;36(4):1841-9.
BACKGROUND/AIM: Indocyanine green (ICG) and the porphyrin precursor 5-aminolevulinic acid (5-ALA) have been approved as fluorescence imaging agents in the clinical setting. This study evaluated the usefulness of fluorescence imaging with both ICG and 5-ALA for intraoperative identification of latent small liver tumors.
2.Role of 5-HT5A and 5-HT1B/1D receptors in the antinociception produced by ergotamine and valerenic acid in the rat formalin test.
Vidal-Cantú GC1, Jiménez-Hernández M2, Rocha-González HI3, Villalón CM1, Granados-Soto V1, Muñoz-Islas E4. Eur J Pharmacol. 2016 Apr 8. pii: S0014-2999(16)30225-4. doi: 10.1016/j.ejphar.2016.04.009. [Epub ahead of print]
Sumatriptan, dihydroergotamine and methysergide inhibit 1% formalin-induced nociception by activation of peripheral 5-HT1B/1D receptors. This study set out to investigate the pharmacological profile of the antinociception produced by intrathecal and intraplantar administration of ergotamine (a 5-HT1B/1D and 5-HT5A/5B receptor agonist) and valerenic acid (a partial agonist at 5-HT5A receptors). Intraplantar injection of 1% formalin in the right hind paw resulted in spontaneous flinching behavior of the injected hindpaw of female Wistar rats. Intrathecal ergotamine (15 nmol) or valerenic acid (1 nmol) blocked in a dose dependent manner formalin-induced nociception. The antinociception by intrathecal ergotamine (15 nmol) or valerenic acid (1 nmol) was partly or completely blocked by intrathecal administration of the antagonists: (i) methiothepin (non-selective 5-HT5A/5B; 0.01-0.1 nmol); (ii) SB-699551 (selective 5-HT5A; up to 10 nmol); (iii) anti-5-HT5A antibody; (iv) SB-224289 (selective 5-HT1B; 0.
3.Treatment of experimental autoimmune uveoretinitis with different natural compounds.
Li M1, Chen X1, Liu J2, Wang D2, Gan L1, Lv X1, Qiao Y2. Mol Med Rep. 2016 Apr 8. doi: 10.3892/mmr.2016.5096. [Epub ahead of print]
Uveitis is an important eye disease that potentially causes loss of sight. Although extensive studies have been conducted on uveitis, the exact pathogenesis remains to be determined. The effects of treatment with natural compounds on an experimental autoimmune uveoretinitis (EAU) rat model were examined in the present study. A total of 25 rats were divided into 5 groups: Alkaloids (n=5), saponins (n=5), flavonoids (n=5), phenols (n=5), and the normal saline group (n=5). The rats in each group were treated with an intraperitoneal injection of proper alkaloids (berberine hydrochloride), saponins (steroidal saponins), flavonoids (baicalein), or phenols (chlorogenic acid) or physiological saline, respectively. The rats' aqueous humour and crystalline lens was then observed under the slit lamp periodically, looking for signs of inflammation. After 2 weeks, the rats were sacrificed and the degree of pathological changes on their eyeballs under different treatment methods were determined using an optical microscope.
4.A Gamma Scintigraphy study to investigate uterine targeting efficiency of Raloxifene loaded Liposomes administered intravaginally in New Zealand white female rabbits.
Patel A1, Tyagi A2, Sharma RK2, Thakkar H1. Drug Deliv. 2016 Apr 12:1-36. [Epub ahead of print]
CONTEXT: Raloxifene Hydrochloride (RLH), a selective estrogen receptor modulator, shows antiproliferative and apoptotic effects on Leiomyoma. Its extensive first pass metabolism leads to oral bioavailability of 2%.