1.N-acetyltransferase activity in the urine in Japanese subjects: comparison in healthy persons and bladder cancer patients.
Ishizu S1, Hashida C, Hanaoka T, Maeda K, Ohishi Y. Jpn J Cancer Res. 1995 Dec;86(12):1179-81.
The activity of urinary N-acetylamino-transferase was determined by high-performance liquid chromatographic assay of acetylisoniazid and isoniazid after administration of isoniazid to healthy Japanese male and bladder cancer patients in Japan. The healthy subjects were 47 college students and 44 company employees ranging from 18 to 64 years old (mean +/- SC = 34.5 +/- 13.7). The bladder cancer group consisted of 58 male and 13 female patients, ranging from 28 to 82 years old (mean +/- SD = 60.8 +/- 11.6), who were being treated at several hospitals. The slow phenotype, defined as an acetylation ratio (acetylisoniazid/isoniazid) of less than 2.0, was observed in 13 (14.3%) of the 91 healthy subjects, and in 20 (28.2%) of the 71 bladder cancer patients; the difference between the two groups is significant (p < 0.05). A histogram of the acetylation ratio values showed an overall leftward shift of the patient group, indicating low values of acetylation ratio in this group as a whole (p < 0.
2.Radiometric studies on the use of selective inhibitors in the identification of Mycobacterium spp.
Collins T1, Levett PN. J Med Microbiol. 1989 Nov;30(3):175-81.
Radiometric selective inhibition tests were developed and evaluated for the rapid differentiation of Mycobacterium spp. Both a p-nitrobenzoic acid (PNB) test and a commercially-prepared p-nitro-alpha-acetylamino-beta-hydroxypropiophenone (NAP) test successfully differentiated M. tuberculosis and M. bovis from "atypical" mycobacteria or mycobacteria other than tubercle bacilli (MOTT). Thiophene-2-carboxylic acid hydrazide (TCH) readily distinguished human M. tuberculosis strains from M. bovis, irrespective of resistance to isoniazid. Both PNB and TCH tests were utilised in a routine radiometric susceptibility testing scheme over a period of 1 year in which 110 isolates of M. tuberculosis, 10 of M. bovis and one isolate of BCG were correctly differentiated from 10 isolates of MOTT. The rapidity, sensitivity and specificity of these radiometric tests can play a useful role in mycobacterial identification.
3.[The acetylator polymorphism in a Khmer population: clinical consequences].
Bechtel YC1, Bechtel PR, Lelouët H, Choisy H, Dy NR. Therapie. 2001 Jul-Aug;56(4):409-13.
The great variability of slow acetylator (SA) and/or rapid acetylator (RA) frequency is mainly due to ethnic-racial origin. Using the urinary elimination ratio of three metabolites of caffeine--acetylamino formylamino methyluracil (AFMU) to AFMU + 1-methyl urate (1U) + 1-methyl xanthine (1X)--we settled the acetylation phenotype in 54 independent subjects of Khmer and 70 independent subjects of Caucasian origin. Using DNA from peripheral leucocytes, we determined by PCR, in 32 Khmer and 122 Caucasian subjects, the frequencies of wild-type alleles (NAT-2 *4) and of mutated alleles (NAT-2 *5A, *6A, *7A). The frequency of SA was respectively 28 per cent and 61 per cent in Khmer and Caucasian subjects. The antimode of the distribution of the ratio was different in the two populations: 0.07 in Khmers and 0.18 in Caucasians showing a reduced acetylation capacity in the Khmer population in spite of a higher frequency of RA. The frequencies of alleles were also different between the two populations.